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    1 条,共 77

    标题: Ectomycorrhizal Cortinarius species participate in enzymatic oxidation of humus in northern forest ecosystems 

    作者: Bodeker, ITM (Bodeker, Inga T. M.); Clemmensen, KE (Clemmensen, Karina E.); de Boer, W (de Boer, Wietse); Martin, F (Martin, Francis); Olson, A (Olson, Ake); Lindahl, BD (Lindahl, Bjorn D.)

    来源出版物: NEW PHYTOLOGIST  : 203  : 1  : 245-256  DOI: 10.1111/nph.12791  出版年: JUL 2014  

    摘要: In northern forests, belowground sequestration of nitrogen (N) in complex organic pools restricts nutrient availability to plants. Oxidative extracellular enzymes produced by ectomycorrhizal fungi may aid plant N acquisition by providing access to N in macromolecular complexes. We test the hypotheses that ectomycorrhizal Cortinarius species produce Mn-dependent peroxidases, and that the activity of these enzymes declines at elevated concentrations of inorganic N.

    In a boreal pine forest and a sub-arctic birch forest, Cortinarius DNA was assessed by 454-sequencing of ITS amplicons and related to Mn-peroxidase activity in humus samples with- and without previous N amendment. Transcription of Cortinarius Mn-peroxidase genes was investigated in field samples. Phylogenetic analyses of Cortinarius peroxidase amplicons and genome sequences were performed.

    We found a significant co-localization of high peroxidase activity and DNA from Cortinarius species. Peroxidase activity was reduced by high ammonium concentrations. Amplification of mRNA sequences indicated transcription of Cortinarius Mn-peroxidase genes under field conditions. The Cortinarius glaucopus genome encodes 11 peroxidases - a number comparable to many white-rot wood decomposers.

    These results support the hypothesis that some ectomycorrhizal fungi - Cortinarius species in particular - may play an important role in decomposition of complex organic matter, linked to their mobilization of organically bound N.

    入藏号: WOS:000336970200024 

    PubMed ID: 24725281 

    作者识别号:

    作者 ResearcherID ORCID

    Library, Library  A-4320-2012  0000-0002-3835-159X 

    de Boer, Wietse  C-2737-2011   

     

    ISSN: 0028-646X 

    eISSN: 1469-8137 

     

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    2 条,共 77

    标题: Metabolic Flux Analysis of Plastidic Isoprenoid Biosynthesis in Poplar Leaves Emitting and Nonemitting Isoprene 

    作者: Ghirardo, A (Ghirardo, Andrea); Wright, LP (Wright, Louwrance Peter); Bi, Z (Bi, Zhen); Rosenkranz, M (Rosenkranz, Maaria); Pulido, P (Pulido, Pablo); Rodriguez-Concepcion, M (Rodriguez-Concepcion, Manuel); Niinemets, U (Niinemets, Uelo); Bruggemann, N (Brueggemann, Nicolas); Gershenzon, J (Gershenzon, Jonathan); Schnitzler, JP (Schnitzler, Joerg-Peter)

    来源出版物: PLANT PHYSIOLOGY  : 165  : 1  : 37-51  DOI: 10.1104/pp.114.236018  出版年: MAY 2014  

    摘要: The plastidic 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway is one of the most important pathways in plants and produces a large variety of essential isoprenoids. Its regulation, however, is still not well understood. Using the stable isotope C-13-labeling technique, we analyzed the carbon fluxes through the MEP pathway and into the major plastidic isoprenoid products in isoprene-emitting and transgenic isoprene-nonemitting (NE) gray poplar (Populus x canescens). We assessed the dependence on temperature, light intensity, and atmospheric [CO2]. Isoprene biosynthesis was by far (99%) the main carbon sink of MEP pathway intermediates in mature gray poplar leaves, and its production required severalfold higher carbon fluxes compared with NE leaves with almost zero isoprene emission. To compensate for the much lower demand for carbon, NE leaves drastically reduced the overall carbon flux within the MEP pathway. Feedback inhibition of 1-deoxy-D-xylulose-5-phosphate synthase activity by accumulated plastidic dimethylallyl diphosphate almost completely explained this reduction in carbon flux. Our data demonstrate that short-term biochemical feedback regulation of 1-deoxy-D-xylulose-5-phosphate synthase activity by plastidic dimethylallyl diphosphate is an important regulatory mechanism of the MEP pathway. Despite being relieved from the large carbon demand of isoprene biosynthesis, NE plants redirected only approximately 0.5% of this saved carbon toward essential nonvolatile isoprenoids, i.e. beta-carotene and lutein, most probably to compensate for the absence of isoprene and its antioxidant properties.

    入藏号: WOS:000335906300004 

    PubMed ID: 24590857 

    作者识别号:

    作者 ResearcherID ORCID

    Brueggemann, Nicolas  C-4263-2014   

    Schnitzler, Joerg-Peter  C-5268-2011  0000-0002-9825-867X 

    Wright, Louwrance  D-9242-2011  0000-0001-5998-6079 

    Niinemets, Ulo  A-3816-2008  0000-0002-3078-2192 

    Gershenzon, Jonathan  K-1331-2013  0000-0002-1812-1551 

    Pulido, Pablo  A-8093-2015  0000-0001-9092-3674 

    Rodriguez-Concepcion, Manuel  I-7229-2015  0000-0002-1280-2305 

     

    ISSN: 0032-0889 

    eISSN: 1532-2548 

     

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    3 条,共 77

    标题: Monolignol Ferulate Transferase Introduces Chemically Labile Linkages into the Lignin Backbone 

    作者: Wilkerson, CG (Wilkerson, C. G.); Mansfield, SD (Mansfield, S. D.); Lu, F (Lu, F.); Withers, S (Withers, S.); Park, JY (Park, J. -Y.); Karlen, SD (Karlen, S. D.); Gonzales-Vigil, E (Gonzales-Vigil, E.); Padmakshan, D (Padmakshan, D.); Unda, F (Unda, F.); Rencoret, J (Rencoret, J.); Ralph, J (Ralph, J.)

    来源出版物: SCIENCE  : 344  : 6179  : 90-93  DOI: 10.1126/science.1250161  出版年: APR 4 2014  

    摘要: Redesigning lignin, the aromatic polymer fortifying plant cell walls, to be more amenable to chemical depolymerization can lower the energy required for industrial processing. We have engineered poplar trees to introduce ester linkages into the lignin polymer backbone by augmenting the monomer pool with monolignol ferulate conjugates. Herein, we describe the isolation of a transferase gene capable of forming these conjugates and its xylem-specific introduction into poplar. Enzyme kinetics, in planta expression, lignin structural analysis, and improved cell wall digestibility after mild alkaline pretreatment demonstrate that these trees produce the monolignol ferulate conjugates, export them to the wall, and use them during lignification. Tailoring plants to use such conjugates during cell wall biosynthesis is a promising way to produce plants that are designed for deconstruction.

    入藏号: WOS:000333746100059 

    PubMed ID: 24700858 

    作者识别号:

    作者 ResearcherID ORCID

    Ralph, John  C-3161-2009   

     

    ISSN: 0036-8075 

    eISSN: 1095-9203 

     

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    4 条,共 77

    标题: Ethylene and jasmonic acid act as negative modulators during mutualistic symbiosis between Laccaria bicolor and Populus roots 

    作者: Plett, JM (Plett, Jonathan M.); Khachane, A (Khachane, Amit); Ouassou, M (Ouassou, Malika); Sundberg, B (Sundberg, Bjorn); Kohler, A (Kohler, Annegret); Martin, F (Martin, Francis)

    来源出版物: NEW PHYTOLOGIST  : 202  : 1  : 270-286  DOI: 10.1111/nph.12655  出版年: APR 2014  

    摘要: The plant hormones ethylene, jasmonic acid and salicylic acid have interconnecting roles during the response of plant tissues to mutualistic and pathogenic symbionts.

    We used morphological studies of transgenic- or hormone-treated Populus roots as well as whole-genome oligoarrays to examine how these hormones affect root colonization by the mutualistic ectomycorrhizal fungus Laccaria bicolor S238N.

    We found that genes regulated by ethylene, jasmonic acid and salicylic acid were regulated in the late stages of the interaction between L. bicolor and poplar. Both ethylene and jasmonic acid treatments were found to impede fungal colonization of roots, and this effect was correlated to an increase in the expression of certain transcription factors (e.g. ETHYLENE RESPONSE FACTOR1) and a decrease in the expression of genes associated with microbial perception and cell wall modification. Further, we found that ethylene and jasmonic acid showed extensive transcriptional cross-talk, cross-talk that was opposed by salicylic acid signaling.

    We conclude that ethylene and jasmonic acid pathways are induced late in the colonization of root tissues in order to limit fungal growth within roots. This induction is probably an adaptive response by the plant such that its growth and vigor are not compromised by the fungus.

    入藏号: WOS:000331737900034 

    PubMed ID: 24383411 

    ISSN: 1469-8137 

     

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    5 条,共 77

    标题: The genome sequence and effector complement of the flax rust pathogen Melampsora lini 

    作者: Nemri, A (Nemri, Adnane); Saunders, DGO (Saunders, Diane G. O.); Anderson, C (Anderson, Claire); Upadhyaya, NM (Upadhyaya, Narayana M.); Win, J (Win, Joe); Lawrence, GJ (Lawrence, Gregory J.); Jones, DA (Jones, David A.); Kamoun, S (Kamoun, Sophien); Ellis, JG (Ellis, Jeffrey G.); Dodds, PN (Dodds, Peter N.)

    来源出版物: FRONTIERS IN PLANT SCIENCE  : 5  文献号: 98  DOI: 10.3389/fpls.2014.00098  出版年: MAR 24 2014  

    摘要: Rust fungi cause serious yield reductions on crops, including wheat, barley, soybean, coffee, and represent real threats to global food security. Of these fungi, the flax rust pathogen Melampsora lint has been developed most extensively over the past 80 years as a model to understand the molecular mechanisms that underpin pathogenesis. During infection, M. lint secretes virulence effectors to promote disease. The number of these effectors, their function and their degree of conservation across rust fungal species is unknown. To assess this, we sequenced and assembled de novo the genome of M. lini isolate CH5 into 21,130 scaffolds spanning 189 Mbp (scaffold N50 of 31 kbp). Global analysis of the DNA sequence revealed that repetitive elements, primarily retrotransposons, make up at least 45% of the genome. Using ab initio predictions, transcriptome data and homology searches, we identified 16,271 putative protein-coding genes. An analysis pipeline was then implemented to predict the effector complement of M. lini and compare it to that of the poplar rust, wheat stem rust and wheat stripe rust pathogens to identify conserved and species-specific effector candidates. Previous knowledge of four cloned M. lint avirulence effector proteins and two basidiomycete effectors was used to optimize parameters of the effector prediction pipeline. Markov clustering based on sequence similarity was performed to group effector candidates from all four rust pathogens. Clusters containing at least one member from M. lint were further analyzed and prioritized based on features including expression in isolated haustoria and infected leaf tissue and conservation across rust species. Herein, we describe 200 of 940 clusters that ranked highest on our priority list, representing 725 flax rust candidate effectors. Our findings on this important model rust species provide insight into how effectors of rust fungi are conserved across species and how they may act to promote infection on their hosts.

    入藏号: WOS:000333248400001 

    PubMed ID: 24715894 

    作者识别号:

    作者 ResearcherID ORCID

    Lawrence, Gregory  D-3926-2009   

    Nemri, Adnane  I-3830-2012   

    Win, Joe  H-2557-2014  0000-0002-9851-2404 

    Jones, David  C-9507-2009   

    Dodds, Peter  D-1181-2009  0000-0003-0620-5923 

    Upadhyaya, Narayana  A-1480-2008  0000-0002-3052-0416 

    Ellis, Jeffrey  A-1999-2010   

    Kamoun, Sophien  B-3529-2009  0000-0002-0290-0315 

     

    ISSN: 1664-462X 

     

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    6 条,共 77

    标题: Geographical and environmental gradients shape phenotypic trait variation and genetic structure in Populus trichocarpa 

    作者: McKown, AD (McKown, Athena D.); Guy, RD (Guy, Robert D.); Klapste, J (Klapste, Jaroslav); Geraldes, A (Geraldes, Armando); Friedmann, M (Friedmann, Michael); Cronk, QCB (Cronk, Quentin C. B.); El-Kassaby, YA (El-Kassaby, Yousry A.); Mansfield, SD (Mansfield, Shawn D.); Douglas, CJ (Douglas, Carl J.)

    来源出版物: NEW PHYTOLOGIST  : 201  : 4  : 1263-1276  DOI: 10.1111/nph.12601  出版年: MAR 2014  

    摘要: Populus trichocarpa is widespread across western North America spanning extensive variation in photoperiod, growing season and climate. We investigated trait variation in P. trichocarpa using over 2000 trees from a common garden at Vancouver, Canada, representing replicate plantings of 461 genotypes originating from 136 provenance localities.

    We measured 40 traits encompassing phenological events, biomass accumulation, growth rates, and leaf, isotope and gas exchange-based ecophysiology traits. With replicated plantings and 29 354 single nucleotide polymorphisms (SNPs) from 3518 genes, we estimated both broad-sense trait heritability (H-2) and overall population genetic structure from principal component analysis.

    Populus trichocarpa had high phenotypic variation and moderate/high H-2 for many traits. H-2 ranged from 0.3 to 0.9 in phenology, 0.3 to 0.8 in biomass and 0.1 to 0.8 in ecophysiology traits. Most traits correlated strongly with latitude, maximum daylength and temperature of tree origin, but not necessarily with elevation, precipitation or heat : moisture indices. Trait H-2 values reflected trait correlation strength with geoclimate variables. The population genetic structure had one significant principal component (PC1) which correlated with daylength and showed enrichment for genes relating to circadian rhythm and photoperiod.

    Robust relationships between traits, population structure and geoclimate in P. trichocarpa reflect patterns which suggest that range-wide geographical and environment gradients have shaped its genotypic and phenotypic variability.

    入藏号: WOS:000338510200022 

    PubMed ID: 24491114 

    ISSN: 0028-646X 

    eISSN: 1469-8137 

     

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    7 条,共 77

    标题: Response of osmolytes in soil to drying and rewetting 

    作者: Warren, CR (Warren, Charles R.)

    来源出版物: SOIL BIOLOGY & BIOCHEMISTRY  : 70  : 22-32  DOI: 10.1016/j.soilbio.2013.12.008  出版年: MAR 2014  

    摘要: The accumulation and subsequent release of microbial osmolytes in response to drying and rewetting are thought to be key players in C and N dynamics, yet studies on soils have failed to support this hypothesis. The aim of this experiment was to determine how low-molecular weight compounds, and osmolytes in particular, are affected by drying and rewetting. Water deficits were imposed slowly by withholding water for 21 weeks from large (200 L) mesocosms vegetated with a globally widespread grass Themeda triandra. A broad spectrum of small molecules in extracts was identified and quantified by capillary electrophoresis-mass spectrometry and gas chromatography-mass spectrometry. Compared with controls, drought-stressed mesocosms contained >10-fold larger amounts of known microbial osmolytes: ectoine, hydroxyectoine, betaine, proline-betaine, trigonelline, proline, trehalose, arabitol. The pool of osmolytes accounted for 3.6% of CHCl3 labile TOC in control mesocosms and 17% of CHCl3 labile TOC in drought-stressed mesocosms. There was no evidence that rewatering led to a large pulse of osmolytes in free solution. Instead osmolytes decreased to control concentrations within 1-3 h of rewatering - probably indicating rapid uptake by microbes and plants. Results of this study suggest that osmolytes can account for a substantial fraction of microbial C, and are at least one of the ways that soil microbes cope with water deficits. (C) 2013 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000332439800004 

    作者识别号:

    作者 ResearcherID ORCID

    Warren, Charles  A-6013-2011  0000-0002-0788-4713 

    Young, Kristina  M-3069-2014   

     

    ISSN: 0038-0717 

     

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    8 条,共 77

    标题: ATTED-II in 2014: Evaluation of Gene Coexpression in Agriculturally Important Plants 

    作者: Obayashi, T (Obayashi, Takeshi); Okamura, Y (Okamura, Yasunobu); Ito, S (Ito, Satoshi); Tadaka, S (Tadaka, Shu); Aoki, Y (Aoki, Yuichi); Shirota, M (Shirota, Matsuyuki); Kinoshita, K (Kinoshita, Kengo)

    来源出版物: PLANT AND CELL PHYSIOLOGY  : 55  : 1  DOI: 10.1093/pcp/pct178  出版年: JAN 2014  

    摘要: ATTED-II (http://atted.jp) is a database of coexpressed genes that was originally developed to identify functionally related genes in Arabidopsis and rice. Herein, we describe an updated version of ATTED-II, which expands this resource to include additional agriculturally important plants. To improve the quality of the coexpression data for Arabidopsis and rice, we included more gene expression data from microarray and RNA sequencing studies. The RNA sequencing-based coexpression data now cover 94% of the Arabidopsis protein-encoding genes, representing a substantial increase from previously available microarray-based coexpression data (76% coverage). We also generated coexpression data for four dicots (soybean, poplar, grape and alfalfa) and one monocot (maize). As both the quantity and quality of expression data for the non-model species are generally poorer than for the model species, we verified coexpression data associated with these new species using multiple methods. First, the overall performance of the coexpression data was evaluated using gene ontology annotations and the coincidence of a genomic feature. Secondly, the reliability of each guide gene was determined by comparing coexpressed gene lists between platforms. With the expanded and newly evaluated coexpression data, ATTED-II represents an important resource for identifying functionally related genes in agriculturally important plants.

    入藏号: WOS:000330448900006 

    PubMed ID: 24334350 

    作者识别号:

    作者 ResearcherID ORCID

    Obayashi, Takeshi  E-9663-2011  0000-0002-3106-722X 

     

    ISSN: 0032-0781 

    eISSN: 1471-9053 

     

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    9 条,共 77

    标题: Comprehensive Genome-Wide Survey, Genomic Constitution and Expression Profiling of the NAC Transcription Factor Family in Foxtail Millet (Setaria italica L.) 

    作者: Puranik, S (Puranik, Swati); Sahu, PP (Sahu, Pranav Pankaj); Mandal, SN (Mandal, Sambhu Nath); Suresh, BV (Suresh, Venkata B.); Parida, SK (Parida, Swarup Kumar); Prasad, M (Prasad, Manoj)

    来源出版物: PLOS ONE  : 8  : 5  文献号: e64594  DOI: 10.1371/journal.pone.0064594  出版年: MAY 15 2013  

    摘要: The NAC proteins represent a major plant-specific transcription factor family that has established enormously diverse roles in various plant processes. Aided by the availability of complete genomes, several members of this family have been identified in Arabidopsis, rice, soybean and poplar. However, no comprehensive investigation has been presented for the recently sequenced, naturally stress tolerant crop, Setaria italica (foxtail millet) that is famed as a model crop for bioenergy research. In this study, we identified 147 putative NAC domain-encoding genes from foxtail millet by systematic sequence analysis and physically mapped them onto nine chromosomes. Genomic organization suggested that inter-chromosomal duplications may have been responsible for expansion of this gene family in foxtail millet. Phylogenetically, they were arranged into 11 distinct sub-families (I-XI), with duplicated genes fitting into one cluster and possessing conserved motif compositions. Comparative mapping with other grass species revealed some orthologous relationships and chromosomal rearrangements including duplication, inversion and deletion of genes. The evolutionary significance as duplication and divergence of NAC genes based on their amino acid substitution rates was understood. Expression profiling against various stresses and phytohormones provides novel insights into specific and/or overlapping expression patterns of SiNAC genes, which may be responsible for functional divergence among individual members in this crop. Further, we performed structure modeling and molecular simulation of a stress-responsive protein, SiNAC128, proffering an initial framework for understanding its molecular function. Taken together, this genome-wide identification and expression profiling unlocks new avenues for systematic functional analysis of novel NAC gene family candidates which may be applied for improvising stress adaption in plants.

    入藏号: WOS:000319052700112 

    PubMed ID: 23691254 

    作者识别号:

    作者 ResearcherID ORCID

    Prasad, Manoj    0000-0003-0691-0163 

     

    ISSN: 1932-6203 

     

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    10 条,共 77

    标题: Effect of ionic liquid/organic solvent pretreatment on the enzymatic hydrolysis of corncob for bioethanol production. Part 1: Structural characterization of the lignins 

    作者: Sun, SN (Sun, Shao-Ni); Li, MF (Li, Ming-Fei); Yuan, TQ (Yuan, Tong-Qi); Xu, F (Xu, Feng); Sun, RC (Sun, Run-Cang)

    来源出版物: INDUSTRIAL CROPS AND PRODUCTS  : 43  : 570-577  DOI: 10.1016/j.indcrop.2012.07.074  出版年: MAY 2013  

    摘要: Corncob was submitted to pretreatments with 1-ethyl-3-methylimadazolium acetate and water/organic solvents (DMSO. DMF, and DMAc) followed by alkaline extraction to isolate lignin. The lignin fractions obtained were comprehensively characterized by alkaline nitrobenzene oxidation, HPAEC, GPC,TGA, UV, FT-IR and HSQC spectra. The results showed that a maximum yield of 85.04% (based on the original lignin) was achieved for L-DMSO prepared with the EMIMAc/DMSO pretreatment. The fractions prepared with EMIMAc/organic solvents contained lower amounts of carbohydrates (0.48-1.40%) than milled wood lignin (MWL, 8.73%) and had similar molecular weights (M-w, 2050-2430 g/mol) to MWL. On the other hand, the fraction L-H20 prepared with EMIMAc/H2O contained relatively abundant carbohydrates (11.19%) and had a high M-w (4310 g/mol) than MWL. HSQC spectra analysis revealed that corncob lignin consisted of beta-O-4, beta-beta, beta-5, beta-1 linkages, and cinnamyl alcohol end groups. In addition, the amounts (Ar/100Ar) of beta-O-4 units increased in the order of L-H2O < L-DMSO < MWL. (C) 2012 Elsevier B.V. All rights reserved.

    入藏号: WOS:000311865000085 

    ISSN: 0926-6690 

     

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    11 条,共 77

    标题: Lignin depolymerization (LDP) in alcohol over nickel-based catalysts via a fragmentation-hydrogenolysis process 

    作者: Song, Q (Song, Qi); Wang, F (Wang, Feng); Cai, JY (Cai, Jiaying); Wang, YH (Wang, Yehong); Zhang, JJ (Zhang, Junjie); Yu, WQ (Yu, Weiqiang); Xu, J (Xu, Jie)

    来源出版物: ENERGY & ENVIRONMENTAL SCIENCE  : 6  : 3  : 994-1007  DOI: 10.1039/c2ee23741e  出版年: MAR 2013  

    摘要: Valorization of native birch wood lignin into monomeric phenols over nickel-based catalysts has been studied. High chemoselectivity to aromatic products was achieved by using Ni-based catalysts and common alcohols as solvents. The results show that lignin can be selectively cleaved into propylguaiacol and propylsyringol with total selectivity >90% at a lignin conversion of about 50%. Alcohols, such as methanol, ethanol and ethylene glycol, are suitable solvents for lignin conversion. Analyses with MALDI-TOF and NMR show that birch lignin is first fragmented into smaller lignin species consisting of several benzene rings with a molecular weight of m/z ca. 1100 to ca. 1600 via alcoholysis reaction. The second step involves the hydrogenolysis of the fragments into phenols. The presence of gaseous H-2 has no effect on lignin conversion, indicating that alcohols provide active hydrogen species, which is further confirmed by isotopic tracing experiments. Catalysts are recycled by magnetic separation and can be reused four times without losing activity. The mechanistic insights from this work could be helpful in understanding native lignin conversion and the formation of monomeric phenolics via reductive depolymerization.

    入藏号: WOS:000315165700036 

    作者识别号:

    作者 ResearcherID ORCID

    xu, jie  E-8159-2014   

     

    ISSN: 1754-5692 

     

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    12 条,共 77

    标题: Fractioning and chemical characterization of barks of Betula pendula and Eucalyptus globulus 

    作者: Miranda, I (Miranda, Isabel); Gominho, J (Gominho, Jorge); Mirra, I (Mirra, Ines); Pereira, H (Pereira, Helena)

    来源出版物: INDUSTRIAL CROPS AND PRODUCTS  : 41  : 299-305  DOI: 10.1016/j.indcrop.2012.04.024  出版年: JAN 2013  

    摘要: The composition of birch (Betula pendula Roth.) and eucalypt (Eucalyptus globulus Labill.) barks was studied after grinding and fractioning into different particles sizes.

    There was a significant difference in the fractionation of both barks in relation to the yield of fines (5.9% and 28.3% of particles under 0.450 for birch and eucalypt, respectively) and of coarser particles over 2 mm (70.7% and 41.4%).

    The chemical composition of birch and eucalypt barks, as a mass weighed average of all granulometric fractions was, respectively: ash 2.9% and 12.1%; total extractives 17.6% and 6.5% (hydrophilic extractives were dominant), lignin 27.9% and 28.8% and holocellulose 49.8% and 62.6%. Birch bark contained a considerable amount of suberin (5.9%) whereas eucalypt bark contained a very small amount (<1%). The carbohydrate composition differed between birch and eucalypt barks, i.e., respectively, glucose 47.0% and 68.4%, and xylose 33.8% and 23.2% of total neutral monosaccharides.

    Ash elemental composition was different in both species. Birch bark contained in relation to eucalypt bark, in the 0.250-0.450 mm fraction, more N (0.69% vs. 0.26%) and P (0.075% vs. 0.001%), and less Ca (0.39% vs. 0.62%), K(0.24% vs. 0.31%) and Mg (0.07% vs. 0.15%). High concentration of Zn was found in birch bark (217 mg/kg vs. 11 mg/kg in eucalypt bark).

    After grinding and granulometric separation, extractives were present preferentially in the finest fraction with an enrichment in dichloromethane and ethanol solubles especially in the case of birch bark. Eucalypt bark had a high content of cellulose and hemicelluloses especially in the coarser fraction. The fibrous character of this fraction shows its potential as a fiber source. (C) 2012 Elsevier B.V. All rights reserved.

    入藏号: WOS:000306681100044 

    作者识别号:

    作者 ResearcherID ORCID

    Gominho, Jorge  B-4816-2009  0000-0003-3419-6075 

    Pereira, Helena    0000-0002-5393-4443 

    Miranda, Isabel    0000-0003-0280-5219 

     

    ISSN: 0926-6690 

     

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    13 条,共 77

    标题: The genetic basis of flowering responses to seasonal cues 

    作者: Andres, F (Andres, Fernando); Coupland, G (Coupland, George)

    来源出版物: NATURE REVIEWS GENETICS  : 13  : 9  : 627-639  DOI: 10.1038/nrg3291  出版年: SEP 2012  

    摘要: Plants respond to the changing seasons to initiate developmental programmes precisely at particular times of year. Flowering is the best characterized of these seasonal responses, and in temperate climates it often occurs in spring. Genetic approaches in Arabidopsis thaliana have shown how the underlying responses to changes in day length (photoperiod) or winter temperature (vernalization) are conferred and how these converge to create a robust seasonal response. Recent advances in plant genome analysis have demonstrated the diversity in these regulatory systems in many plant species, including several crops and perennials, such as poplar trees. Here, we report progress in defining the diverse genetic mechanisms that enable plants to recognize winter, spring and autumn to initiate flower development.

    入藏号: WOS:000308064000010 

    PubMed ID: 22898651 

    ISSN: 1471-0056 

     

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    14 条,共 77

    标题: Characterization of Slow Pyrolysis Biochars: Effects of Feedstocks and Pyrolysis Temperature on Biochar Properties 

    作者: Kloss, S (Kloss, Stefanie); Zehetner, F (Zehetner, Franz); Dellantonio, A (Dellantonio, Alex); Hamid, R (Hamid, Raad); Ottner, F (Ottner, Franz); Liedtke, V (Liedtke, Volker); Schwanninger, M (Schwanninger, Manfred); Gerzabek, MH (Gerzabek, Martin H.); Soja, G (Soja, Gerhard)

    来源出版物: JOURNAL OF ENVIRONMENTAL QUALITY  : 41  : 4  : 990-1000  DOI: 10.2134/jeq2011.0070  出版年: JUL 2012  

    摘要: Biochars are increasingly used as soil amendment and for C sequestration in soils. The influence of feedstock differences and pyrolysis temperature on biochar characteristics has been widely studied. However, there is a lack of knowledge about the formation of potentially toxic compounds that remain in the biochars after pyrolysis. We investigated biochars from three feedstocks (wheat straw, poplar wood, and spruce wood) that were slowly pyrolyzed at 400, 460, and 525 degrees C for 5 h (straw) and 10 h (woodchips), respectively. We characterized the biochars' pH, electrical conductivity, elemental composition (by dry combustion and X-ray fluorescence), surface area (by N-2 adsorption), water-extractable major elements, and cation exchange capacity (CEC). We further conducted differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffractometry to obtain information on the biochars' molecular characteristics and mineralogical composition. We investigated trace metal content, total polycyclic aromatic hydrocarbon (PAH) content, and PAH composition in the biochars. The highest salt (4.92 mS cm(-1)) and ash (12.7%) contents were found in straw-derived biochars. The H/C ratios of biochars with highest treatment temperature (HTT) 525 degrees C were 0.46 to 0.40. Surface areas were low but increased (1.8-56 m(2) g(-1)) with increasing HTT, whereas CEC decreased (162-52 mmol(c) kg(-1)) with increasing HTT. Th e results of DSC and FTIR suggested a loss of labile, aliphatic compounds during pyrolysis and the formation of more recalcitrant, aromatic constituents. X-ray diffractometry patterns indicated a mineralogical restructuring of biochars with increasing HTT. Water-extractable major and trace elements varied considerably with feedstock composition, with trace elements also affected by HTT. Total PAH contents (sum of EPA 16 PAHs) were highly variable with values up to 33.7 mg kg(-1); irrespective of feedstock type, the composition of PAHs showed increasing dominance of naphthalene with increasing HTT. Th e results demonstrate that biochars are highly heterogeneous materials that, depending on feedstock and HTT, may be suitable for soil application by contributing to the nutrient status and adding recalcitrant C to the soil but also potentially pose ecotoxicological challenges.

    入藏号: WOS:000305966900003 

    PubMed ID: 22751041 

    作者识别号:

    作者 ResearcherID ORCID

    Soja, Gerhard  A-9657-2008   

    Schwanninger, Manfred  E-6420-2010  0000-0002-5822-9129 

     

    ISSN: 0047-2425 

     

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    15 条,共 77

    标题: Ethanol production from poplar wood through enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments 

    作者: Wang, ZJ (Wang, Z. J.); Zhu, JY (Zhu, J. Y.); Zalesny, RS (Zalesny, Ronald S., Jr.); Chen, KF (Chen, K. F.)

    来源出版物: FUEL  : 95  : 1  : 606-614  DOI: 10.1016/j.fuel.2011.12.032  出版年: MAY 2012  

    摘要: Dilute acid (DA) and Sulfite Pretreatment to Overcome Recalcitrance of Lignocelluloses (SPORL) pretreatments were directly applied to wood chips of four poplar wood samples of different genotypes (hereafter referred to as poplars; Populus tremuloides Michx. 'native aspen collection'; Populus deltoides Bartr. ex Marsh x Populus nigra L. 'NE222' and 'DN5'; P. nigra x Populus maximowiczii A. Henry 'NM6') to evaluate their bioconversion potential. Plant biomass recalcitrance (PBR) was defined to quantitatively determine the recalcitrance of the poplars. Using DA pretreatment, NM6 produced the lowest bioconversion efficiency with a total monomeric sugar yield of 18% theoretical and an ethanol yield of 0.07 L kg (1) of wood compared with an aspen sugar yield of 47% theoretical and an ethanol yield of 0.17 L kg (1) of wood. Similar comparisons following SPORL pretreatment were 43% versus 55% and 0.11 versus 0.20 L kg (1) of wood for NM6 and aspen, respectively. Bioconversion performance of NE222 and DN5 fell between that of aspen and NM6. While substrate lignin content and lignin removal by pretreatments did not affect substrate enzymatic digestibility, the wood lignin content was found to negatively affect xylan or hemicellulose removal using both DA and SPORL pretreatments. The ability of lignin protecting hemicellulose removal dictates PBR through affecting disk milling energy for size reduction of pretreated wood chips, substrate enzymatic digestibility. The SPORL pretreatment not only improved sugar and ethanol yields over DA for all four poplars, but also better dealt with the differences among them, suggesting better tolerance to feedstock variability. Published by Elsevier Ltd.

    入藏号: WOS:000300615900079 

    ISSN: 0016-2361 

     

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    16 条,共 77

    标题: Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper 

    作者: Choi, DS (Choi, Du Seok); Hwang, IS (Hwang, In Sun); Hwang, BK (Hwang, Byung Kook)

    来源出版物: PLANT CELL  : 24  : 4  : 1675-1690  DOI: 10.1105/tpc.112.095869  出版年: APR 2012  

    摘要: Plants recruit innate immune receptors such as leucine-rich repeat (LRR) proteins to recognize pathogen attack and activate defense genes. Here, we identified the pepper (Capsicum annuum) pathogenesis-related protein10 (PR10) as a leucine-rich repeat protein1 (LRR1)-interacting partner. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the specific interaction between LRR1 and PR10 in planta. Avirulent Xanthomonas campestris pv vesicatoria infection induces PR10 expression associated with the hypersensitive cell death response. Transient expression of PR10 triggers hypersensitive cell death in pepper and Nicotiana benthamiana leaves, which is amplified by LRR1 coexpression as a positive regulator. LRR1 promotes the ribonuclease activity and phosphorylation of PR10, leading to enhanced cell death signaling. The LRR1-PR10 complex is formed in the cytoplasm, resulting in its secretion into the apoplastic space. Engineered nuclear confinement of both proteins revealed that the cytoplasmic localization of the PR10-LRR1 complex is essential for cell death-mediated defense signaling. PR10/LRR1 silencing in pepper compromises resistance to avirulent X. campestris pv vesicatoria infection. By contrast, PR10/LRR1 overexpression in Arabidopsis thaliana confers enhanced resistance to Pseudomonas syringae pv tomato and Hyaloperonospora arabidopsidis. Together, these results suggest that the cytosolic LRR-PR10 complex is responsible for cell death-mediated defense signaling.

    入藏号: WOS:000304595500028 

    PubMed ID: 22492811 

    作者识别号:

    作者 ResearcherID ORCID

    Choi, Duseok  E-6467-2015  0000-0002-2120-055X 

     

    ISSN: 1040-4651 

     

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    17 条,共 77

    标题: A Revised Architecture of Primary Cell Walls Based on Biomechanical Changes Induced by Substrate-Specific Endoglucanases 

    作者: Park, YB (Park, Yong Bum); Cosgrove, DJ (Cosgrove, Daniel J.)

    来源出版物: PLANT PHYSIOLOGY  : 158  : 4  : 1933-1943  DOI: 10.1104/pp.111.192880  出版年: APR 2012  

    摘要: Xyloglucan is widely believed to function as a tether between cellulose microfibrils in the primary cell wall, limiting cell enlargement by restricting the ability of microfibrils to separate laterally. To test the biomechanical predictions of this "tethered network" model, we assessed the ability of cucumber (Cucumis sativus) hypocotyl walls to undergo creep (long-term, irreversible extension) in response to three family-12 endo-beta-1,4-glucanases that can specifically hydrolyze xyloglucan, cellulose, or both. Xyloglucan-specific endoglucanase (XEG from Aspergillus aculeatus) failed to induce cell wall creep, whereas an endoglucanase that hydrolyzes both xyloglucan and cellulose (Cel12A from Hypocrea jecorina) induced a high creep rate. A cellulose-specific endoglucanase (CEG from Aspergillus niger) did not cause cell wall creep, either by itself or in combination with XEG. Tests with additional enzymes, including a family-5 endoglucanase, confirmed the conclusion that to cause creep, endoglucanases must cut both xyloglucan and cellulose. Similar results were obtained with measurements of elastic and plastic compliance. Both XEG and Cel12A hydrolyzed xyloglucan in intact walls, but Cel12A could hydrolyze a minor xyloglucan compartment recalcitrant to XEG digestion. Xyloglucan involvement in these enzyme responses was confirmed by experiments with Arabidopsis (Arabidopsis thaliana) hypocotyls, where Cel12A induced creep in wild-type but not in xyloglucan-deficient (xxt1/xxt2) walls. Our results are incompatible with the common depiction of xyloglucan as a load-bearing tether spanning the 20- to 40-nm spacing between cellulose microfibrils, but they do implicate a minor xyloglucan component in wall mechanics. The structurally important xyloglucan may be located in limited regions of tight contact between microfibrils.

    入藏号: WOS:000303001400035 

    PubMed ID: 22362871 

    作者识别号:

    作者 ResearcherID ORCID

    Yi, Hojae  B-2972-2008   

     

    ISSN: 0032-0889 

     

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    18 条,共 77

    标题: Designed for deconstruction - poplar trees altered in cell wall lignification improve the efficacy of bioethanol production 

    作者: Mansfield, SD (Mansfield, Shawn D.); Kang, KY (Kang, Kyu-Young); Chapple, C (Chapple, Clint)

    来源出版物: NEW PHYTOLOGIST  : 194  : 1  特刊: SI  : 91-101  DOI: 10.1111/j.1469-8137.2011.04031.x  出版年: APR 2012  

    摘要: There is a pressing global need to reduce the increasing societal reliance on petroleum and to develop a bio-based economy. At the forefront is the need to establish a sustainable, renewable, alternative energy sector. This includes liquid transportation fuel derived from lignocellulosic plant materials. However, one of the current limiting factors restricting the effective and efficient conversion of lignocellulosic residues is the recalcitrance of the substrate to enzymatic conversion. In an attempt to assess the impact of cell wall lignin on recalcitrance, we subjected poplar trees engineered with altered lignin content and composition to two potential industrial pretreatment regimes, and evaluated the overall efficacy of the bioconversion to ethanol process. It was apparent that total lignin content has a greater impact than monomer ratio (syringyl : guaiacyl) on both pretreatments. More importantly, low lignin plants showed as much as a 15% improvement in the efficiency of conversion, with near complete hydrolysis of the cellulosic polymer. Using genomic tools to breed or select for modifications in key cell wall chemical and/or ultrastructural traits can have a profound effect on bioenergy processing. These techniques may therefore offer means to overcome the current obstacles that underpin the recalcitrance of lignocellulosic substrates to bioconversion.

    入藏号: WOS:000300719400012 

    PubMed ID: 22239166 

    ISSN: 0028-646X 

     

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    19 条,共 77

    标题: The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus 

    作者: Li, EY (Li, Eryang); Bhargava, A (Bhargava, Apurva); Qiang, WY (Qiang, Weiya); Friedmann, MC (Friedmann, Michael C.); Forneris, N (Forneris, Natascha); Savidge, RA (Savidge, Rodney A.); Johnson, LA (Johnson, Lee A.); Mansfield, SD (Mansfield, Shawn D.); Ellis, BE (Ellis, Brian E.); Douglas, CJ (Douglas, Carl J.)

    来源出版物: NEW PHYTOLOGIST  : 194  : 1  特刊: SI  : 102-115  DOI: 10.1111/j.1469-8137.2011.04016.x  出版年: APR 2012  

    摘要: The formation of secondary cell walls in cell types such as tracheary elements and fibers is a defining characteristic of vascular plants. The Arabidopsis transcription factor KNAT7 is a component of a transcription network that regulates secondary cell wall biosynthesis, but its function has remained unclear. We conducted anatomical, biochemical and molecular phenotypic analyses of Arabidopsis knat7 loss-of-function alleles, KNAT7 over-expression lines and knat7 lines expressing poplar KNAT7. KNAT7 was strongly expressed in concert with secondary wall formation in Arabidopsis and poplar. Arabidopsis knat7 loss-of-function alleles exhibited irregular xylem phenotypes, but also showed increased secondary cell wall thickness in fibers. Increased commitment to secondary cell wall biosynthesis was accompanied by increased lignin content and elevated expression of secondary cell wall biosynthetic genes. KNAT7 over-expression resulted in thinner interfascicular fiber cell walls. Taken together with data demonstrating that KNAT7 is a transcriptional repressor, we hypothesize that KNAT7 is a negative regulator of secondary wall biosynthesis, and functions in a negative feedback loop that represses metabolically inappropriate commitment to secondary wall formation, thereby maintaining metabolic homeostasis. The conservation of the KNAT7 regulatory module in poplar suggests new ways to manipulate secondary cell wall deposition for improvement of bioenergy traits in this tree.

    入藏号: WOS:000300719400013 

    PubMed ID: 22236040 

    作者识别号:

    作者 ResearcherID ORCID

    Douglas, Carl  B-1384-2013   

     

    ISSN: 0028-646X 

     

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    20 条,共 77

    标题: Plant-borne flavonoids released into the rhizosphere: impact on soil bio-activities related to plant nutrition. A review 

    作者: Cesco, S (Cesco, S.); Mimmo, T (Mimmo, T.); Tonon, G (Tonon, G.); Tomasi, N (Tomasi, N.); Pinton, R (Pinton, R.); Terzano, R (Terzano, R.); Neumann, G (Neumann, G.); Weisskopf, L (Weisskopf, L.); Renella, G (Renella, G.); Landi, L (Landi, L.); Nannipieri, P (Nannipieri, P.)

    来源出版物: BIOLOGY AND FERTILITY OF SOILS  : 48  : 2  : 123-149  DOI: 10.1007/s00374-011-0653-2  出版年: FEB 2012  

    摘要: Plants produce and release in the surrounding soil, the so-called rhizosphere, a vast variety of secondary metabolites. Among them, flavonoids are the most studied, mainly for their role in the establishment of rhizobium-legume symbiosis; on the other hand, some studies highlight that they are also important in the plant strategies to acquire nutrients from the soil, for example, by acting on its chemistry. The scope of this review is to give a quick overview on the types and amounts of plant-released flavonoids in order to focus on their effects on soil activities that in turn can influence nutrient availability and so plant mineral nutrition; emphasis is given to the different nutrient cycles, soil enzyme, and soil bacteria activities, and their influence on soil macrofauna and roots of other plants. Finally, the possible outcome of the climate change on these processes is discussed.

    入藏号: WOS:000301384900001 

    作者识别号:

    作者 ResearcherID ORCID

    Cesco, Stefano  F-3088-2011   

    Tomasi, Nicola  A-7505-2012  0000-0002-2136-7720 

    Terzano, Roberto    0000-0001-6232-6151 

     

    ISSN: 0178-2762 

     

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    21 条,共 77

    标题: MYB46 and MYB83 Bind to the SMRE Sites and Directly Activate a Suite of Transcription Factors and Secondary Wall Biosynthetic Genes 

    作者: Zhong, RQ (Zhong, Ruiqin); Ye, ZH (Ye, Zheng-Hua)

    来源出版物: PLANT AND CELL PHYSIOLOGY  : 53  : 2  : 368-380  DOI: 10.1093/pcp/pcr185  出版年: FEB 2012  

    摘要: MYB46 and MYB83 are two functionally redundant Arabidopsis thaliana MYB transcription factors that act as master switches regulating secondary wall biosynthesis. Here, we report the identification of the transcriptional responsive elements and global analysis of the direct targets of MYB46 and MYB83. Using the estrogen-inducible direct activation system, we found that a number of previously identified MYB46 downstream transcription factors, including MYB43, MYB52, MYB54, MYB58, MYB63 and KNAT7, are direct targets of MYB46. Promoter deletion coupled with transactivation analysis of the MYB63 promoter led to the identification of a 7 bp sequence that is sufficient to be responsive to MYB46 activation, and therefore this sequence is designated as the secondary wall MYB-responsive element (SMRE). Further single nucleotide mutation together with electrophoretic mobility shift assay mapped the SMRE consensus sequence as ACC(A/T)A(A/C)(T/C). Genome-wide analysis of direct targets of MYB46 demonstrated that it directly regulates the expression of not only a number of downstream transcription factors, but also a suite of secondary wall biosynthetic genes, some of which are also directly activated by secondary wall NAC (SWN) master switches or by MYB46 direct targets. Furthermore, MYB83 was found to bind to the same SMRE consensus sequence and activate the same set of direct targets as MYB46. Our study has revealed that the transcription program regulating secondary wall biosynthesis involves a multileveled feed-forward loop regulatory structure in which MYB46/MYB83 together with their regulators SWNs and their direct targets regulate an array of downstream genes thereby activating the secondary wall biosynthetic program.

    入藏号: WOS:000300497500011 

    PubMed ID: 22197883 

    ISSN: 0032-0781 

     

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    22 条,共 77

    标题: Phytoextraction of toxic metals: a central role for glutathione 

    作者: Seth, CS (Seth, C. S.); Remans, T (Remans, T.); Keunen, E (Keunen, E.); Jozefczak, M (Jozefczak, M.); Gielen, H (Gielen, H.); Opdenakker, K (Opdenakker, K.); Weyens, N (Weyens, N.); Vangronsveld, J (Vangronsveld, J.); Cuypers, A (Cuypers, A.)

    来源出版物: PLANT CELL AND ENVIRONMENT  : 35  : 2  特刊: SI  : 334-346  DOI: 10.1111/j.1365-3040.2011.02338.x  : FEB 2012  

    摘要: Phytoextraction has a promising potential as an environmentally friendly clean-up method for soils contaminated with toxic metals. To improve the development of efficient phytoextraction strategies, better knowledge regarding metal uptake, translocation and detoxification in planta is a prerequisite. This review highlights our current understanding on these mechanisms, and their impact on plant growth and health. Special attention is paid to the central role of glutathione (GSH) in this process. Because of the high affinity of metals to thiols and as a precursor for phytochelatins (PCs), GSH is an essential metal chelator. Being an important antioxidant, a direct link between metal detoxification and the oxidative challenge in plants growing on contaminated soils is observed, where GSH could be a key player. In addition, as redox couple, oxidized and reduced GSH transmits specific information, in this way tuning cellular signalling pathways under environmental stress conditions. Possible improvements of phytoextraction could be achieved by using transgenic plants or plant-associated microorganisms. Joined efforts should be made to cope with the challenges faced with phytoextraction in order to successfully implement this technique in the field.

    入藏号: WOS:000298795600012 

    PubMed ID: 21486307 

    作者识别号:

    作者 ResearcherID ORCID

    Cuypers, Ann    0000-0002-0171-0245 

     

    ISSN: 0140-7791 

    eISSN: 1365-3040 

     

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    23 条,共 77

    标题: Using Hierarchical Clustering of Secreted Protein Families to Classify and Rank Candidate Effectors of Rust Fungi 

    作者: Saunders, DGO (Saunders, Diane G. O.); Win, J (Win, Joe); Cano, LM (Cano, Liliana M.); Szabo, LJ (Szabo, Les J.); Kamoun, S (Kamoun, Sophien); Raffaele, S (Raffaele, Sylvain)

    来源出版物: PLOS ONE  : 7  : 1  文献号: e29847  DOI: 10.1371/journal.pone.0029847  出版年: JAN 6 2012  

    摘要: Rust fungi are obligate biotrophic pathogens that cause considerable damage on crop plants. Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, and Melampsora larici-populina, the poplar leaf rust pathogen, have strong deleterious impacts on wheat and poplar wood production, respectively. Filamentous pathogens such as rust fungi secrete molecules called disease effectors that act as modulators of host cell physiology and can suppress or trigger host immunity. Current knowledge on effectors from other filamentous plant pathogens can be exploited for the characterisation of effectors in the genome of recently sequenced rust fungi. We designed a comprehensive in silico analysis pipeline to identify the putative effector repertoire from the genome of two plant pathogenic rust fungi. The pipeline is based on the observation that known effector proteins from filamentous pathogens have at least one of the following properties: (i) contain a secretion signal, (ii) are encoded by in planta induced genes, (iii) have similarity to haustorial proteins, (iv) are small and cysteine rich, (v) contain a known effector motif or a nuclear localization signal, (vi) are encoded by genes with long intergenic regions, (vii) contain internal repeats, and (viii) do not contain PFAM domains, except those associated with pathogenicity. We used Markov clustering and hierarchical clustering to classify protein families of rust pathogens and rank them according to their likelihood of being effectors. Using this approach, we identified eight families of candidate effectors that we consider of high value for functional characterization. This study revealed a diverse set of candidate effectors, including families of haustorial expressed secreted proteins and small cysteine-rich proteins. This comprehensive classification of candidate effectors from these devastating rust pathogens is an initial step towards probing plant germplasm for novel resistance components.

    入藏号: WOS:000301188800042 

    PubMed ID: 22238666 

    作者识别号:

    作者 ResearcherID ORCID

    Raffaele, Sylvain  F-7187-2012   

    Kamoun, Sophien  B-3529-2009  0000-0002-0290-0315 

    Cano, Liliana  D-2093-2013   

    Win, Joe  H-2557-2014  0000-0002-9851-2404 

     

    ISSN: 1932-6203 

     

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    24 条,共 77

    标题: The Effects of Tropospheric Ozone on Net Primary Productivity and Implications for Climate Change 

    作者: Ainsworth, EA (Ainsworth, Elizabeth A.); Yendrek, CR (Yendrek, Craig R.); Sitch, S (Sitch, Stephen); Collins, WJ (Collins, William J.); Emberson, LD (Emberson, Lisa D.)

    编者: Merchant SS

    来源出版物: ANNUAL REVIEW OF PLANT BIOLOGY, VOL 63  丛书: Annual Review of Plant Biology  : 63  : 637-661  DOI: 10.1146/annurev-arplant-042110-103829  出版年: 2012  

    摘要: Tropospheric ozone (O-3) is a global air pollutant that causes billions of dollars in lost plant productivity annually. It is an important anthropogenic greenhouse gas, and as a secondary air pollutant, it is present at high concentrations in rural areas far from industrial sources. It also reduces plant productivity by entering leaves through the stomata, generating other reactive oxygen species and causing oxidative stress, which in turn decreases photosynthesis, plant growth, and biomass accumulation. The deposition of O-3 into vegetation through stomata is an important sink for tropospheric O-3 , but this sink is modified by other aspects of environmental change, including rising atmospheric carbon dioxide concentrations, rising temperature, altered precipitation, and nitrogen availability. We review the atmospheric chemistry governing tropospheric O-3 mass balance, the effects of O-3 on stomatal conductance and net primary productivity, and implications for agriculture, carbon sequestration, and climate change.

    入藏号: WOS:000307953100026 

    PubMed ID: 22404461 

    作者识别号:

    作者 ResearcherID ORCID

    Collins, William  A-5895-2010  0000-0002-7419-0850 

    Yendrek, Craig  A-7126-2013  0000-0002-0557-325X 

    sasmito, sigit  E-8715-2013  0000-0001-5864-8596 

    Sitch, Stephen  F-8034-2015   

     

    ISSN: 1543-5008 

    ISBN: 978-0-8243-0663-2

     

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    25 条,共 77

    标题: Characterization of Lignin Structures and Lignin-Carbohydrate Complex (LCC) Linkages by Quantitative C-13 and 2D HSQC NMR Spectroscopy 

    作者: Yuan, TQ (Yuan, Tong-Qi); Sun, SN (Sun, Shao-Ni); Xu, F (Xu, Feng); Sun, RC (Sun, Run-Cang)

    来源出版物: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY  : 59  : 19  : 10604-10614  DOI: 10.1021/jf2031549  出版年: OCT 12 2011  

    摘要: To characterize the lignin structures and lignin-carbohydrate complex (LCC) linkages, milled wood lignin (MWL) and mild acidolysis lignin (MAL) with a high content of associated carbohydrates were sequentially isolated from ball-milled poplar wood. Quantification of their structural features has been achieved by using a combination of quantitative C-13 and 2D HSQC NMR techniques. The results showed that acetylated 4-O-methylgluconoxylan is the main carbohydrate associated with lignins, and acetyl groups frequently acylate the C2 and C3 positions. MWL and MAL exhibited similar structural features. The main substructures were beta-O-4' aryl ether, resinol, and phenylcoumaran, and their abundances per 100 Ar units changed from 41.5 to 43.3, from 14.6 to 12.7, and from 3.7 to 4.0, respectively. The S/G ratios were estimated to be 1.57 and 1.62 for MWL and MAL, respectively. Phenyl glycoside and benzyl ether LCC linkages were clearly quantified, whereas the amount of gamma-ester LCC linkages was ambiguous for quantification.

    入藏号: WOS:000295488000027 

    PubMed ID: 21879769 

    ISSN: 0021-8561 

     

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    26 条,共 77

    标题: A White Spruce Gene Catalog for Conifer Genome Analyses 

    作者: Rigault, P (Rigault, Philippe); Boyle, B (Boyle, Brian); Lepage, P (Lepage, Pierre); Cooke, JEK (Cooke, Janice E. K.); Bousquet, J (Bousquet, Jean); MacKay, JJ (MacKay, John J.)

    来源出版物: PLANT PHYSIOLOGY  : 157  : 1  : 14-28  DOI: 10.1104/pp.111.179663  出版年: SEP 2011  

    摘要: Several angiosperm plant genomes, including Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), poplar (Populus trichocarpa), and grapevine (Vitis vinifera), have been sequenced, but the lack of reference genomes in gymnosperm phyla reduces our understanding of plant evolution and restricts the potential impacts of genomics research. A gene catalog was developed for the conifer tree Picea glauca (white spruce) through large-scale expressed sequence tag sequencing and full-length cDNA sequencing to facilitate genome characterizations, comparative genomics, and gene mapping. The resource incorporates new and publicly available sequences into 27,720 cDNA clusters, 23,589 of which are represented by full-length insert cDNAs. Expressed sequence tags, mate-pair cDNA clone analysis, and custom sequencing were integrated through an iterative process to improve the accuracy of clustering outcomes. The entire catalog spans 30 Mb of unique transcribed sequence. We estimated that the P. glauca nuclear genome contains up to 32,520 transcribed genes owing to incomplete, partially sequenced, and unsampled transcripts and that its transcriptome could span up to 47 Mb. These estimates are in the same range as the Arabidopsis and rice transcriptomes. Next-generation methods confirmed and enhanced the catalog by providing deeper coverage for rare transcripts, by extending many incomplete clusters, and by augmenting the overall transcriptome coverage to 38 Mb of unique sequence. Genomic sample sequencing at 8.5% of the 19.8-Gb P. glauca genome identified 1,495 clusters representing highly repeated sequences among the cDNA clusters. With a conifer transcriptome in full view, functional and protein domain annotations clearly highlighted the divergences between conifers and angiosperms, likely reflecting their respective evolutionary paths.

    入藏号: WOS:000294491800002 

    PubMed ID: 21730200 

    作者识别号:

    作者 ResearcherID ORCID

    Cooke, Janice   G-5906-2011  0000-0002-4990-628X 

    MacKay, John  M-6978-2014  0000-0002-4883-195X 

     

    ISSN: 0032-0889 

     

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    27 条,共 77

    标题: A Secreted Effector Protein of Laccaria bicolor Is Required for Symbiosis Development 

    作者: Plett, JM (Plett, Jonathan M.); Kemppainen, M (Kemppainen, Minna); Kale, SD (Kale, Shiv D.); Kohler, A (Kohler, Annegret); Legue, V (Legue, Valerie); Brun, A (Brun, Annick); Tyler, BM (Tyler, Brett M.); Pardo, AG (Pardo, Alejandro G.); Martin, F (Martin, Francis)

    来源出版物: CURRENT BIOLOGY  : 21  : 14  : 1197-1203  DOI: 10.1016/j.cub.2011.05.033  出版年: JUL 26 2011  

    摘要: Soil-borne mutualistic fungi, such as the ectomycorrhizal fungi, have helped shape forest communities worldwide over the last 180 million years through a mutualistic relationship with tree roots in which the fungal partner provides a large array of nutrients to the plant host in return for photosynthetically derived sugars [1, 2]. This exchange is essential for continued growth and productivity of forest trees, especially in nutrient-poor soils. To date, the signals from the two partners that mediate this symbiosis have remained uncharacterized. Here we demonstrate that MYCORRHIZAL INDUCED SMALL SECRETED PROTEIN 7 (MiSSP7), the most highly symbiosis-upregulated gene from the ectomycorrhizal fungus Laccaria bicolor [3], encodes an effector protein indispensible for the establishment of mutualism. MiSSP7 is secreted by the fungus upon receipt of diffusible signals from plant roots, imported into the plant cell via phosphatidylinositol 3-phosphate-mediated endocytosis, and targeted to the plant nucleus where it alters the transcriptome of the plant cell. L. bicolor transformants with reduced expression of MiSSP7 do not enter into symbiosis with poplar roots. MiSSP7 resembles effectors of pathogenic fungi, nematodes, and bacteria that are similarly targeted to the plant nucleus to promote colonization of the plant tissues [4-9] and thus can be considered a mutualism effector.

    入藏号: WOS:000293320000020 

    PubMed ID: 21757352 

    ISSN: 0960-9822 

     

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    28 条,共 77

    标题: Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica 

    作者: Li, BS (Li, Bosheng); Qin, YR (Qin, Yurong); Duan, H (Duan, Hui); Yin, WL (Yin, Weilun); Xia, XL (Xia, Xinli)

    来源出版物: JOURNAL OF EXPERIMENTAL BOTANY  : 62  : 11  : 3765-3779  DOI: 10.1093/jxb/err051  出版年: JUL 2011  

    摘要: MicroRNAs (miRNAs) are small, non-coding RNAs that play essential roles in plant growth, development, and stress response. Populus euphratica is a typical abiotic stress-resistant woody species. This study presents an efficient method for genome-wide discovery of new drought stress responsive miRNAs in P. euphratica. High-throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 58 new miRNAs belonging to 38 families were identified, an increase in the number of P. euphratica miRNAs. Twenty-six new and 21 conserved miRNA targets were verified by degradome sequencing, and target annotation showed that these targets were involved in multiple biological processes, including transcriptional regulation and response to stimulus. Furthermore, comparison of high-throughput sequencing with miRNA microarray profiling data indicated that 104 miRNA sequences were up-regulated, whereas 27 were down-regulated under drought stress. This preliminary characterization provides a framework for future analysis of miRNA genes and their roles in key poplar traits such as stress resistance, and could be useful for plant breeding and environmental protection.

    入藏号: WOS:000292838700007 

    PubMed ID: 21511902 

    ISSN: 0022-0957 

     

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    29 条,共 77

    标题: FLOWERING LOCUS T duplication coordinates reproductive and vegetative growth in perennial poplar 

    作者: Hsu, CY (Hsu, Chuan-Yu); Adams, JP (Adams, Joshua P.); Kim, HJ (Kim, Hyejin); No, K (No, Kyoungok); Ma, CP (Ma, Caiping); Strauss, SH (Strauss, Steven H.); Drnevich, J (Drnevich, Jenny); Vandervelde, L (Vandervelde, Lindsay); Ellis, JD (Ellis, Jeffrey D.); Rice, BM (Rice, Brandon M.); Wickett, N (Wickett, Norman); Gunter, LE (Gunter, Lee E.); Tuskan, GA (Tuskan, Gerald A.); Brunner, AM (Brunner, Amy M.); Page, GP (Page, Grier P.); Barakat, A (Barakat, Abdelali); Carlson, JE (Carlson, John E.); dePamphilis, CW (dePamphilis, Claude W.); Luthe, DS (Luthe, Dawn S.); Yuceer, C (Yuceer, Cetin)

    来源出版物: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA  : 108  : 26  : 10756-10761  DOI: 10.1073/pnas.1104713108  出版年: JUN 28 2011  

    摘要: Annual plants grow vegetatively at early developmental stages and then transition to the reproductive stage, followed by senescence in the same year. In contrast, after successive years of vegetative growth at early ages, woody perennial shoot meristems begin repeated transitions between vegetative and reproductive growth at sexual maturity. However, it is unknown how these repeated transitions occur without a developmental conflict between vegetative and reproductive growth. We report that functionally diverged paralogs FLOWERING LOCUS T1 (FT1) and FLOWERING LOCUS T2 (FT2), products of whole-genome duplication and homologs of Arabidopsis thaliana gene FLOWERING LOCUS T (FT), coordinate the repeated cycles of vegetative and reproductive growth in woody perennial poplar (Populus spp.). Our manipulative physiological and genetic experiments coupled with field studies, expression profiling, and network analysis reveal that reproductive onset is determined by FT1 in response to winter temperatures, whereas vegetative growth and inhibition of bud set are promoted by FT2 in response to warm temperatures and long days in the growing season. The basis for functional differentiation between FT1 and FT2 appears to be expression pattern shifts, changes in proteins, and divergence in gene regulatory networks. Thus, temporal separation of reproductive onset and vegetative growth into different seasons via FT1 and FT2 provides seasonality and demonstrates the evolution of a complex perennial adaptive trait after genome duplication.

    入藏号: WOS:000292251000074 

    PubMed ID: 21653885 

    ISSN: 0027-8424 

     

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    30 条,共 77

    标题: Quantification of lignin-carbohydrate linkages with high-resolution NMR spectroscopy 

    作者: Balakshin, M (Balakshin, Mikhail); Capanema, E (Capanema, Ewellyn); Gracz, H (Gracz, Hanna); Chang, HM (Chang, Hou-min); Jameel, H (Jameel, Hasan)

    来源出版物: PLANTA  : 233  : 6  : 1097-1110  DOI: 10.1007/s00425-011-1359-2  出版年: JUN 2011  

    摘要: A quantitative approach to characterize lignin-carbohydrate complex (LCC) linkages using a combination of quantitative (13)C NMR and HSQC 2D NMR techniques has been developed. Crude milled wood lignin (MWLc), LCC extracted from MWLc with acetic acid (LCC-AcOH) and cellulolytic enzyme lignin (CEL) preparations were isolated from loblolly pine (Pinus taeda) and white birch (Betula pendula) woods and characterized using this methodology on a routine 300 MHz NMR spectrometer and on a 950 MHz spectrometer equipped with a cryogenic probe. Structural variations in the pine and birch LCC preparations of different types (MWL, CEL and LCC-AcOH) were elucidated. The use of the high field NMR spectrometer equipped with the cryogenic probe resulted in a remarkable improvement in the resolution of the LCC signals and, therefore, is of primary importance for an accurate quantification of LCC linkages. The preparations investigated showed the presence of different amounts of benzyl ether, gamma-ester and phenyl glycoside LCC bonds. Benzyl ester moieties were not detected. Pine LCC-AcOH and birch MWLc preparations were preferable for the analysis of phenyl glycoside and ester LCC linkages in pine and birch, correspondingly, whereas CEL preparations were the best to study benzyl ether LCC structures. The data obtained indicate that pinewood contains higher amounts of benzyl ether LCC linkages, but lower amounts of phenyl glycoside and gamma-ester LCC moieties as compared to birch wood.

    入藏号: WOS:000291037900003 

    PubMed ID: 21298285 

    ISSN: 0032-0935 

     

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    31 条,共 77

    标题: Lignin content in natural Populus variants affects sugar release 

    作者: Studer, MH (Studer, Michael H.); DeMartini, JD (DeMartini, Jaclyn D.); Davis, MF (Davis, Mark F.); Sykes, RW (Sykes, Robert W.); Davison, B (Davison, Brian); Keller, M (Keller, Martin); Tuskan, GA (Tuskan, Gerald A.); Wyman, CE (Wyman, Charles E.)

    来源出版物: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA  : 108  : 15  : 6300-6305  DOI: 10.1073/pnas.1009252108  出版年: APR 12 2011  

    摘要: The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin content and ratio of syringyl and guaiacyl units (S/G ratio). This subset was tested for total sugar release through enzymatic hydrolysis alone as well as through combined hot-water pretreatment and enzymatic hydrolysis using a high-throughput screening method. The total amount of glucan and xylan released varied widely among samples, with total sugar yields of up to 92% of the theoretical maximum. A strong negative correlation between sugar release and lignin content was only found for pretreated samples with an S/G ratio < 2.0. For higher S/G ratios, sugar release was generally higher, and the negative influence of lignin was less pronounced. When examined separately, only glucose release was correlated with lignin content and S/G ratio in this manner, whereas xylose release depended on the S/G ratio alone. For enzymatic hydrolysis without pretreatment, sugar release increased significantly with decreasing lignin content below 20%, irrespective of the S/G ratio. Furthermore, certain samples featuring average lignin content and S/G ratios exhibited exceptional sugar release. These facts suggest that factors beyond lignin and S/G ratio influence recalcitrance to sugar release and point to a critical need for deeper understanding of cell-wall structure before plants can be rationally engineered for reduced recalcitrance and efficient biofuels production.

    入藏号: WOS:000289413600072 

    PubMed ID: 21444820 

    作者识别号:

    作者 ResearcherID ORCID

    Keller, Martin  C-4416-2012   

    Davison, Brian  D-7617-2013  0000-0002-7408-3609 

     

    ISSN: 0027-8424 

     

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    32 条,共 77

    标题: Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass 

    作者: Kim, Y (Kim, Youngmi); Ximenes, E (Ximenes, Eduardo); Mosier, NS (Mosier, Nathan S.); Ladisch, MR (Ladisch, Michael R.)

    来源出版物: ENZYME AND MICROBIAL TECHNOLOGY  : 48  : 4-5  : 408-415  DOI: 10.1016/j.enzmictec.2011.01.007  出版年: APR 7 2011  

    摘要: Liquid hot water, steam explosion, and dilute acid pretreatments of lignocellulose generate soluble inhibitors which hamper enzymatic hydrolysis as well as fermentation of sugars to ethanol. Toxic and inhibitory compounds will vary with pretreatment and include soluble sugars, furan derivatives (hydroxymethyl fulfural, furfural), organic acids (acetic, formic and, levulinic acid), and phenolic compounds. Their effect is seen when an increase in the concentration of pretreated biomass in a hydrolysis slurry results in decreased cellulose conversion, even though the ratio of enzyme to cellulose is kept constant. We used lignin-free cellulose, Solka Floc, combined with mixtures of soluble components released during pretreatment of wood, to prove that the decrease in the rate and extent of cellulose hydrolysis is due to a combination of enzyme inhibition and deactivation. The causative agents were extracted from wood pretreatment liquid using PEG surfactant, activated charcoal or ethyl acetate and then desorbed, recovered, and added back to a mixture of enzyme and cellulose. At enzyme loadings of either 1 or 25 mg protein/g glucan, the most inhibitory components, later identified as phenolics, decreased the rate and extent of cellulose hydrolysis by half due to both inhibition and precipitation of the enzymes. Full enzyme activity occurred when the phenols were removed. Hence detoxification of pretreated woods through phenol removal is expected to reduce enzyme loadings, and therefore reduce enzyme costs, for a given level of cellulose conversion. (C) 2011 Elsevier Inc. All rights reserved.

    入藏号: WOS:000289930900017 

    PubMed ID: 22112958 

    作者识别号:

    作者 ResearcherID ORCID

    Ximenes, Eduardo  G-5323-2013   

     

    ISSN: 0141-0229 

     

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    33 条,共 77

    标题: Pseudo-lignin and pretreatment chemistry 

    作者: Sannigrahi, P (Sannigrahi, Poulomi); Kim, DH (Kim, Dong Ho); Jung, S (Jung, Seokwon); Ragauskas, A (Ragauskas, Arthur)

    来源出版物: ENERGY & ENVIRONMENTAL SCIENCE  : 4  : 4  : 1306-1310  DOI: 10.1039/c0ee00378f  出版年: APR 2011  

    摘要: The formation of pseudo-lignin by the combination of carbohydrate and lignin degradation products has been proposed to be responsible for the increased Klason lignin content in biomass pretreated under acidic conditions. Direct evidence for the presence of pseudo-lignin has never been presented. The formation of additional lignin-like material may be detrimental to enzymatic hydrolysis due to the non-productive binding of enzymes with lignin. To investigate the chemistry of pseudo-lignin formation, dilute acid pretreatments were performed on delignified hybrid poplar biomass under conditions of varying severity. The results show a progressive increase in the Klason lignin content of the acid pretreated material with increasing pretreatment severity. NMR and FT-IR spectroscopic characterization shows the development of aliphatic, unsaturated and carbonyl carbon functionalities in the samples pretreated at higher severities. Given the very low Klason lignin content of the starting material, acid catalyzed dehydration of carbohydrates is responsible for the formation of pseudo-lignin.

    入藏号: WOS:000289001400022 

    ISSN: 1754-5692 

     

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    34 条,共 77

    标题: Individualization of cellulose nanofibers from wood using high-intensity ultrasonication combined with chemical pretreatments 

    作者: Chen, WS (Chen, Wenshuai); Yu, HP (Yu, Haipeng); Liu, YX (Liu, Yixing); Chen, P (Chen, Peng); Zhang, MX (Zhang, Mingxin); Hai, YF (Hai, Yunfei)

    来源出版物: CARBOHYDRATE POLYMERS  : 83  : 4  : 1804-1811  DOI: 10.1016/j.carbpol.2010.10.040  出版年: FEB 1 2011  

    摘要: Cellulose nanofibers were individualized from poplar wood in two distinct stages. Initially, wood fibers were subjected to a chemical process to eliminate lignin and hemicellulose. The obtained chemical-purified cellulose fibers were then mechanically separated into nanofibers using high-intensity ultrasonication. The diameter distributions of the resulting nanofibers were dependent on the output power of the ultrasonic treatment. TEM and FE-SEM images showed that the diameter of the obtained nanofibers ranged from 5 to 20 nm when the output power of the conducted ultrasonication was greater than 1000W. FTIR and XRD results indicated that hemicellulose and lignin were removed extensively in the cellulose nanofibers, with a crystallinity of approximately 69%. The TGA results showed that the degradation temperature of the nanofibers was dramatically increased to approximately 335 degrees C compared with 210 degrees C of the original wood fibers. The obtained nanofibers may be potentially applied in various fields, such as bio-nanocomposites, tissue engineering scaffolds, filtration media, packaging, and so on. (C) 2010 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000286965400049 

    作者识别号:

    作者 ResearcherID ORCID

    Liu, Yifei  L-7828-2014  0000-0002-1087-9827 

     

    ISSN: 0144-8617 

     

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    35 条,共 77

    标题: Chilling of Dormant Buds Hyperinduces FLOWERING LOCUS T and Recruits GA-Inducible 1,3-beta-Glucanases to Reopen Signal Conduits and Release Dormancy in Populus 

    作者: Rinne, PLH (Rinne, Paivi L. H.); Welling, A (Welling, Annikki); Vahala, J (Vahala, Jorma); Ripel, L (Ripel, Linda); Ruonala, R (Ruonala, Raili); Kangasjarvi, J (Kangasjarvi, Jaakko); van der Schoot, C (van der Schoot, Christiaan)

    来源出版物: PLANT CELL  : 23  : 1  : 130-146  DOI: 10.1105/tpc.110.081307  出版年: JAN 2011  

    摘要: In trees, production of intercellular signals and accessibility of signal conduits jointly govern dormancy cycling at the shoot apex. We identified 10 putative cell wall 1,3-beta-glucanase genes (glucan hydrolase family 17 [ GH17]) in Populus that could turn over 1,3-beta-glucan (callose) at pores and plasmodesmata (PD) and investigated their regulation in relation to FT and CENL1 expression. The 10 genes encode orthologs of Arabidopsis thaliana BG_ppap, a PD-associated glycosylphosphatidylinositol (GPI) lipid-anchored protein, the Arabidopsis PD callose binding protein PDCB, and a birch (Betula pendula) putative lipid body (LB) protein. We found that these genes were differentially regulated by photoperiod, by chilling (5 degrees C), and by feeding of gibberellins GA(3) and GA(4). GA(3) feeding upregulated all LB-associated GH17s, whereas GA(4) upregulated most GH17s with a GPI anchor and/or callose binding motif, but only GA(4) induced true bud burst. Chilling upregulated a number of GA biosynthesis and signaling genes as well as FT, but not CENL1, while the reverse was true for both GA(3) and GA(4). Collectively, the results suggest a model for dormancy release in which chilling induces FT and both GPI lipid-anchored and GA(3)-inducible GH17s to reopen signaling conduits in the embryonic shoot. When temperatures rise, the reopened conduits enable movement of FT and CENL1 to their targets, where they drive bud burst, shoot elongation, and morphogenesis.

    入藏号: WOS:000287860300013 

    PubMed ID: 21282527 

    作者识别号:

    作者 ResearcherID ORCID

    Kangasjarvi, Jaakko  A-3024-2015   

     

    ISSN: 1040-4651 

     

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    36 条,共 77

    标题: Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation 

    作者: Zhu, JY (Zhu, J. Y.); Pan, XJ (Pan, X. J.)

    来源出版物: BIORESOURCE TECHNOLOGY  : 101  : 13  特刊: SI  : 4992-5002  DOI: 10.1016/j.biortech.2009.11.007  出版年: JUL 2010  

    摘要: This review presents a comprehensive discussion of the key technical issues in woody biomass pretreatment: barriers to efficient cellulose saccharification, pretreatment energy consumption, in particular energy consumed for wood-size reduction, and criteria to evaluate the performance of a pretreatment. A post-chemical pretreatment size-reduction approach is proposed to significantly reduce mechanical energy consumption. Because the ultimate goal of biofuel production is net energy output, a concept of pretreatment energy efficiency (kg/MJ) based on the total sugar recovery (kg/kg wood) divided by the energy consumption in pretreatment (MJ/kg wood) is defined. It is then used to evaluate the performances of three of the most promising pretreatment technologies: steam explosion, organosolv, and sulfite pretreatment to overcome lignocelluloses recalcitrance (SPORL) for softwood pretreatment. The present study found that SPORL is the most efficient process and produced highest sugar yield. Other important issues, such as the effects of lignin on substrate saccharification and the effects of pretreatment on high-value lignin utilization in woody biomass pretreatment, are also discussed. Published by Elsevier Ltd.

    入藏号: WOS:000276867500033 

    PubMed ID: 19969450 

    作者识别号:

    作者 ResearcherID ORCID

    mei, chaoqun  B-9857-2011    

     

    ISSN: 0960-8524 

     

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    37 条,共 77

    标题: Genome-Wide Classification and Evolutionary Analysis of the bHLH Family of Transcription Factors in Arabidopsis, Poplar, Rice, Moss, and Algae 

    作者: Carretero-Paulet, L (Carretero-Paulet, Lorenzo); Galstyan, A (Galstyan, Anahit); Roig-Villanova, I (Roig-Villanova, Irma); Martinez-Garcia, JF (Martinez-Garcia, Jaime F.); Bilbao-Castro, JR (Bilbao-Castro, Jose R.); Robertson, DL (Robertson, David L.)

    来源出版物: PLANT PHYSIOLOGY  : 153  : 3  : 1398-1412  DOI: 10.1104/pp.110.153593  出版年: JUL 2010  

    摘要: Basic helix-loop-helix proteins (bHLHs) are found throughout the three eukaryotic kingdoms and constitute one of the largest families of transcription factors. A growing number of bHLH proteins have been functionally characterized in plants. However, some of these have not been previously classified. We present here an updated and comprehensive classification of the bHLHs encoded by the whole sequenced genomes of Arabidopsis (Arabidopsis thaliana), Populus trichocarpa, Oryza sativa, Physcomitrella patens, and five algae species. We define a plant bHLH consensus motif, which allowed the identification of novel highly diverged atypical bHLHs. Using yeast two-hybrid assays, we confirm that (1) a highly diverged bHLH has retained protein interaction activity and (2) the two most conserved positions in the consensus play an essential role in dimerization. Phylogenetic analysis permitted classification of the 638 bHLH genes identified into 32 subfamilies. Evolutionary and functional relationships within subfamilies are supported by intron patterns, predicted DNA-binding motifs, and the architecture of conserved protein motifs. Our analyses reveal the origin and evolutionary diversification of plant bHLHs through differential expansions, domain shuffling, and extensive sequence divergence. At the functional level, this would translate into different subfamilies evolving specific DNA-binding and protein interaction activities as well as differential transcriptional regulatory roles. Our results suggest a role for bHLH proteins in generating plant phenotypic diversity and provide a solid framework for further investigations into the role carried out in the transcriptional regulation of key growth and developmental processes.

    入藏号: WOS:000279400200040 

    PubMed ID: 20472752 

    作者识别号:

    作者 ResearcherID ORCID

    ROIG VILLANOVA, IRMA  K-3865-2014  0000-0001-9124-0429 

     

    ISSN: 0032-0889 

     

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    38 条,共 77

    标题: Genome-Wide Analysis of Ethylene-Responsive Element Binding Factor-Associated Amphiphilic Repression Motif-Containing Transcriptional Regulators in Arabidopsis 

    作者: Kagale, S (Kagale, Sateesh); Links, MG (Links, Matthew G.); Rozwadowski, K (Rozwadowski, Kevin)

    来源出版物: PLANT PHYSIOLOGY  : 152  : 3  : 1109-1134  DOI: 10.1104/pp.109.151704  出版年: MAR 2010  

    摘要: The ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif is a transcriptional regulatory motif identified in members of the ethylene-responsive element binding factor, C2H2, and auxin/indole-3-acetic acid families of transcriptional regulators. Sequence comparison of the core EAR motif sites from these proteins revealed two distinct conservation patterns: LxLxL and DLNxxP. Proteins containing these motifs play key roles in diverse biological functions by negatively regulating genes involved in developmental, hormonal, and stress signaling pathways. Through a genome-wide bioinformatics analysis, we have identified the complete repertoire of the EAR repressome in Arabidopsis (Arabidopsis thaliana) comprising 219 proteins belonging to 21 different transcriptional regulator families. Approximately 72% of these proteins contain a LxLxL type of EAR motif, 22% contain a DLNxxP type of EAR motif, and the remaining 6% have a motif where LxLxL and DLNxxP are overlapping. Published in vitro and in planta investigations support approximately 40% of these proteins functioning as negative regulators of gene expression. Comparative sequence analysis of EAR motif sites and adjoining regions has identified additional preferred residues and potential posttranslational modification sites that may influence the functionality of the EAR motif. Homology searches against protein databases of poplar (Populus trichocarpa), grapevine (Vitis vinifera), rice (Oryza sativa), and sorghum (Sorghum bicolor) revealed that the EAR motif is conserved across these diverse plant species. This genome-wide analysis represents the most extensive survey of EAR motif-containing proteins in Arabidopsis to date and provides a resource enabling investigations into their biological roles and the mechanism of EAR motif-mediated transcriptional regulation.

    入藏号: WOS:000276329500001 

    PubMed ID: 20097792 

    作者识别号:

    作者 ResearcherID ORCID

    Links, Matthew  K-6624-2012   

    Kagale, Sateesh    0000-0002-7213-1590 

     

    ISSN: 0032-0889 

     

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    39 条,共 77

    标题: Cross-Kingdom Comparison of Transcriptomic Adjustments to Low-Oxygen Stress Highlights Conserved and Plant-Specific Responses 

    作者: Mustroph, A (Mustroph, Angelika); Lee, SC (Lee, Seung Cho); Oosumi, T (Oosumi, Teruko); Zanetti, ME (Eugenia Zanetti, Maria); Yang, HJ (Yang, Huijun); Ma, K (Ma, Kelvin); Yaghoubi-Masihi, A (Yaghoubi-Masihi, Arbi); Fukao, T (Fukao, Takeshi); Bailey-Serres, J (Bailey-Serres, Julia)

    来源出版物: PLANT PHYSIOLOGY  : 152  : 3  : 1484-1500  DOI: 10.1104/pp.109.151845  出版年: MAR 2010   

    摘要: High-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, or complete submergence. We showed previously that Arabidopsis (Arabidopsis thaliana) seedlings elevate the levels of hundreds of transcripts, including a core group of 49 genes that are prioritized for translation across cell types of both shoots and roots. To recognize low-oxygen responses that are evolutionarily conserved versus species specific, we compared the transcriptomic reconfiguration in 21 organisms from four kingdoms (Plantae, Animalia, Fungi, and Bacteria). Sorting of organism proteomes into clusters of putative orthologs identified broadly conserved responses associated with glycolysis, fermentation, alternative respiration, metabolite transport, reactive oxygen species amelioration, chaperone activity, and ribosome biogenesis. Differentially regulated genes involved in signaling and transcriptional regulation were poorly conserved across kingdoms. Strikingly, nearly half of the induced mRNAs of Arabidopsis seedlings encode proteins of unknown function, of which over 40% had up-regulated orthologs in poplar (Populus trichocarpa), rice (Oryza sativa), or Chlamydomonas reinhardtii. Sixteen HYPOXIA-RESPONSIVE UNKNOWN PROTEIN (HUP) genes, including four that are Arabidopsis specific, were ectopically overexpressed and evaluated for their effect on seedling tolerance to oxygen deprivation. This allowed the identification of HUPs coregulated with genes associated with anaerobic metabolism and other processes that significantly enhance or reduce stress survival when ectopically overexpressed. These findings illuminate both broadly conserved and plant-specific low-oxygen stress responses and confirm that plant-specific HUPs with limited phylogenetic distribution influence low-oxygen stress endurance.

    入藏号: WOS:000276329500030 

    PubMed ID: 20097791 

    作者识别号:

    作者 ResearcherID ORCID

    Bailey-Serres, Julia    0000-0002-8568-7125 

     

    ISSN: 0032-0889 

     

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    40 条,共 77

    标题: Functional Characterization of Poplar Wood-Associated NAC Domain Transcription Factors 

    作者: Zhong, RQ (Zhong, Ruiqin); Lee, CH (Lee, Chanhui); Ye, ZH (Ye, Zheng-Hua)

    来源出版物: PLANT PHYSIOLOGY  : 152  : 2  : 1044-1055  DOI: 10.1104/pp.109.148270  出版年: FEB 2010  

    摘要: Wood is the most abundant biomass produced by land plants. Dissection of the molecular mechanisms underlying the transcriptional regulation of wood formation is a fundamental issue in plant biology and has important implications in tree biotechnology. Although a number of transcription factors in tree species have been shown to be associated with wood formation and some of them are implicated in lignin biosynthesis, none of them have been demonstrated to be key regulators of the biosynthesis of all three major components of wood. In this report, we have identified a group of NAC domain transcription factors, PtrWNDs, that are preferentially expressed in developing wood of poplar (Populus trichocarpa). Expression of PtrWNDs in the Arabidopsis (Arabidopsis thaliana) snd1 nst1 double mutant effectively complemented the secondary wall defects in fibers, indicating that PtrWNDs are capable of activating the entire secondary wall biosynthetic program. Overexpression of PtrWND2B and PtrWND6B in Arabidopsis induced the expression of secondary wall-associated transcription factors and secondary wall biosynthetic genes and, concomitantly, the ectopic deposition of cellulose, xylan, and lignin. Furthermore, PtrWND2B and PtrWND6B were able to activate the promoter activities of a number of poplar wood-associated transcription factors and wood biosynthetic genes. Together, these results demonstrate that PtrWNDs are functional orthologs of SND1 and suggest that PtrWNDs together with their downstream transcription factors form a transcriptional network involved in the regulation of wood formation in poplar.

    入藏号: WOS:000274246600053 

    PubMed ID: 19965968 

    ISSN: 0032-0889 

     

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    41 条,共 77

    标题: Genome sequence of the palaeopolyploid soybean 

    作者: Schmutz, J (Schmutz, Jeremy); Cannon, SB (Cannon, Steven B.); Schlueter, J (Schlueter, Jessica); Ma, JX (Ma, Jianxin); Mitros, T (Mitros, Therese); Nelson, W (Nelson, William); Hyten, DL (Hyten, David L.); Song, QJ (Song, Qijian); Thelen, JJ (Thelen, Jay J.); Cheng, JL (Cheng, Jianlin); Xu, D (Xu, Dong); Hellsten, U (Hellsten, Uffe); May, GD (May, Gregory D.); Yu, Y (Yu, Yeisoo); Sakurai, T (Sakurai, Tetsuya); Umezawa, T (Umezawa, Taishi); Bhattacharyya, MK (Bhattacharyya, Madan K.); Sandhu, D (Sandhu, Devinder); Valliyodan, B (Valliyodan, Babu); Lindquist, E (Lindquist, Erika); Peto, M (Peto, Myron); Grant, D (Grant, David); Shu, SQ (Shu, Shengqiang); Goodstein, D (Goodstein, David); Barry, K (Barry, Kerrie); Futrell-Griggs, M (Futrell-Griggs, Montona); Abernathy, B (Abernathy, Brian); Du, JC (Du, Jianchang); Tian, ZX (Tian, Zhixi); Zhu, LC (Zhu, Liucun); Gill, N (Gill, Navdeep); Joshi, T (Joshi, Trupti); Libault, M (Libault, Marc); Sethuraman, A (Sethuraman, Anand); Zhang, XC (Zhang, Xue-Cheng); Shinozaki, K (Shinozaki, Kazuo); Nguyen, HT (Nguyen, Henry T.); Wing, RA (Wing, Rod A.); Cregan, P (Cregan, Perry); Specht, J (Specht, James); Grimwood, J (Grimwood, Jane); Rokhsar, D (Rokhsar, Dan); Stacey, G (Stacey, Gary); Shoemaker, RC (Shoemaker, Randy C.); Jackson, SA (Jackson, Scott A.)

    来源出版物: NATURE  : 463  : 7278  : 178-183  DOI: 10.1038/nature08670  出版年: JAN 14 2010  

    摘要: Soybean (Glycine max) is one of the most important crop plants for seed protein and oil content, and for its capacity to fix atmospheric nitrogen through symbioses with soil-borne microorganisms. We sequenced the 1.1-gigabase genome by a whole-genome shotgun approach and integrated it with physical and high-density genetic maps to create a chromosome-scale draft sequence assembly. We predict 46,430 protein-coding genes, 70% more than Arabidopsis and similar to the poplar genome which, like soybean, is an ancient polyploid (palaeopolyploid). About 78% of the predicted genes occur in chromosome ends, which comprise less than one-half of the genome but account for nearly all of the genetic recombination. Genome duplications occurred at approximately 59 and 13 million years ago, resulting in a highly duplicated genome with nearly 75% of the genes present in multiple copies. The two duplication events were followed by gene diversification and loss, and numerous chromosome rearrangements. An accurate soybean genome sequence will facilitate the identification of the genetic basis of many soybean traits, and accelerate the creation of improved soybean varieties.

    入藏号: WOS:000273582700025 

    PubMed ID: 20075913 

    作者识别号:

    作者 ResearcherID ORCID

    Umezawa, Taishi  E-3028-2010  0000-0003-3750-0503 

    Shinozaki, Kazuo  G-4202-2013   

    Schmutz, Jeremy  N-3173-2013   

    Sakurai, Tetsuya  B-2690-2010   

    Cheng, Jianlin  N-8209-2013   

     

    ISSN: 0028-0836 

     

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    42 条,共 77

    标题: Flowering time control and applications in plant breeding 

    作者: Jung, C (Jung, Christian); Muller, AE (Mueller, Andreas E.)

    来源出版物: TRENDS IN PLANT SCIENCE  : 14  : 10  : 563-573  DOI: 10.1016/j.tplants.2009.07.005  出版年: OCT 2009  

    摘要: Shifting the seasonal timing of reproduction is a major goal of plant breeding efforts to produce novel varieties that are better adapted to local environments and changing climatic conditions. The key regulators of floral transition have been studied extensively in model species, and in recent years a growing number of related genes have been identified in crop species, with some notable exceptions. These sequences and variants thereof, as well as several major genes which were only identified in crop species, can now be used by breeders as molecular markers and for targeted genetic modification of flowering time. This article reviews the major floral regulatory pathways and discusses current and novel strategies for altering bolting and flowering behavior in crop plants.

    入藏号: WOS:000271054200007 

    PubMed ID: 19716745 

    作者识别号:

    作者 ResearcherID ORCID

    Muller, Andreas  C-3590-2012  0000-0002-2746-5030 

     

    ISSN: 1360-1385 

     

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    43 条,共 77

    标题: Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies 

    作者: Kumar, R (Kumar, Rajeev); Mago, G (Mago, Gaurav); Balan, V (Balan, Venkatesh); Wyman, CE (Wyman, Charles E.)

    来源出版物: BIORESOURCE TECHNOLOGY  : 100  : 17  : 3948-3962  DOI: 10.1016/j.biortech.2009.01.075  出版年: SEP 2009  

    摘要: In order to investigate changes in substrate chemical and physical features after pretreatment, several characterizations were performed on untreated (LIT) corn stover and poplar and their solids resulting pretreatments by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, flowthrough, lime, and SO(2) technologies. In addition to measuring the chemical compositions including acetyl content, physical attributes determined were biomass crystallinity, cellulose degree of polymerization, cellulase adsorption capacity of pretreated solids and enzymatically extracted lignin, copper number, FT-IR responses, scanning electron microscopy (SEM) visualizations, and surface atomic composition by electron spectroscopy of chemical analysis (ESCA). Lime pretreatment removed the most acetyl groups from both corn stover and poplar, while AFEX removed the least. Low pH pretreatments depolymerized cellulose and enhanced biomass crystallinity much more than higher pH approaches. Lime pretreated corn stover solids and flowthrough pretreated poplar solids had the highest cellulase adsorption capacity, while dilute acid pretreated corn stover solids and controlled pH pretreated poplar solids had the least. Furthermore, enzymatically extracted AFEX lignin preparations for both corn stover and poplar had the lowest cellulase adsorption capacity. ESCA results showed that SO(2) pretreated solids had the highest surface O/C ratio for poplar, but for corn stover, the highest value was observed for dilute acid pretreatment with a Parr reactor. Although dependent on pretreatment and substrate, FT-IR data showed that along with changes in cross linking and chemical changes, pretreatments may also decrystallize cellulose and change the ratio of crystalline cellulose polymorphs (I alpha/I beta). (C) 2009 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000266752200018 

    PubMed ID: 19362819 

    作者识别号:

    作者 ResearcherID ORCID

    Kumar, Rajeev  B-9236-2011   

    mago, gaurav  A-6753-2013  0000-0003-2316-8458 

    Mago, Gaurav  I-3356-2013   

    Kumar, Rajeev    0000-0001-7523-0108 

     

    ISSN: 0960-8524 

     

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    44 条,共 77

    标题: Sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure 

    作者: Coleman, HD (Coleman, Heather D.); Yan, J (Yan, Jimmy); Mansfield, SD (Mansfield, Shawn D.)

    来源出版物: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA  : 106  : 31  : 13118-13123  DOI: 10.1073/pnas.0900188106  出版年: AUG 4 2009  

    摘要: Overexpression of the Gossypium hirsutum sucrose synthase (SuSy) gene under the control of 2 promoters was examined in hybrid poplar (Populus alba x grandidentata). Analysis of RNA transcript abundance, enzyme activity, cell wall composition, and soluble carbohydrates revealed significant changes in the transgenic lines. All lines showed significantly increased SuSy enzyme activity in developing xylem. This activity manifested in altered secondary cell wall cellulose content per dry weight in all lines, with increases of 2% to 6% over control levels, without influencing plant growth. The elevated concentration of cellulose was associated with an increase in cell wall crystallinity but did not alter secondary wall microfibril angle. This finding suggests that the observed increase in crystallinity is a function of altered carbon partitioning to cellulose biosynthesis rather than the result of tension wood formation. Furthermore, the augmented deposition of cellulose in the transgenic lines resulted in thicker xylem secondary cell wall and consequently improved wood density. These findings clearly implicate SuSy as a key regulator of sink strength in poplar trees and demonstrate the tight association of SuSy with cellulose synthesis and secondary wall formation.

    入藏号: WOS:000268667600098 

    PubMed ID: 19625620 

    作者识别号:

    作者 ResearcherID ORCID

    Coleman, Heather    0000-0002-4923-601X 

     

    ISSN: 0027-8424 

     

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    45 条,共 77

    标题: The Effects on Lignin Structure of Overexpression of Ferulate 5-Hydroxylase in Hybrid Poplar 

    作者: Stewart, JJ (Stewart, Jaclyn J.); Akiyama, T (Akiyama, Takuya); Chapple, C (Chapple, Clint); Ralph, J (Ralph, John); Mansfield, SD (Mansfield, Shawn D.)

    来源出版物: PLANT PHYSIOLOGY  : 150  : 2  : 621-635  DOI: 10.1104/pp.109.137059  出版年: JUN 2009  

    摘要: Poplar (Populus tremula X alba) lignins with exceedingly high syringyl monomer levels are produced by overexpression of the ferulate 5-hydroxylase (F5H) gene driven by a cinnamate 4-hydroxylase (C4H) promoter. Compositional data derived from both standard degradative methods and NMR analyses of the entire lignin component (as well as isolated lignin fraction) indicated that the C4H::F5H transgenic's lignin was comprised of as much as 97.5% syringyl units (derived from sinapyl alcohol), the remainder being guaiacyl units (derived from coniferyl alcohol); the syringyl level in the wild-type control was 68%. The resultant transgenic lignins are more linear and display a lower degree of polymerization. Although the crucial beta-ether content is similar, the distribution of other interunit linkages in the lignin polymer is markedly different, with higher resinol (beta-beta) and spirodienone (beta-1) contents, but with virtually no phenylcoumarans (beta-5, which can only be formed from guaiacyl units). p-Hydroxybenzoates, acylating the gamma-positions of lignin side chains, were reduced by >50%, suggesting consequent impacts on related pathways. A model depicting the putative structure of the transgenic lignin resulting from the overexpression of F5H is presented. The altered structural features in the transgenic lignin polymer, as revealed here, support the contention that there are significant opportunities to improve biomass utilization by exploiting the malleability of plant lignification processes.

    入藏号: WOS:000266663700009 

    PubMed ID: 19386808 

    作者识别号:

    作者 ResearcherID ORCID

    Ralph, John  C-3161-2009   

     

    ISSN: 0032-0889 

     

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    46 条,共 77

    标题: Cell wall features with regard to mechanical performance. A review COST Action E35 2004-2008: Wood machining - micromechanics and fracture 

    作者: Salmen, L (Salmen, Lennart); Burgert, I (Burgert, Ingo)

    来源出版物: HOLZFORSCHUNG  : 63  : 2  : 121-129  DOI: 10.1515/HF.2009.011  出版年: MAR 2009   

    摘要: The mechanical performance of wood and wood products is highly dependent on the structural arrangement and properties of the polymers within the fibre cell wall. To improve utilisation and manufacture of wood materials, there is an increasing need for a more detailed knowledge regarding structure/property relations at the micro- or nanostructural level. In this article, recent developments regarding our understanding of the wood cell wall structure and its mechanical performance are summarised. The new results are interpreted in relation to property performances of wood fibres and wood tissues. Suggestions are made for future requirements for research in this field.

    入藏号: WOS:000263932200002 

    ISSN: 0018-3830 

     

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    47 条,共 77

    标题: Expansion and Diversification of the Populus R2R3-MYB Family of Transcription Factors 

    作者: Wilkins, O (Wilkins, Olivia); Nahal, H (Nahal, Hardeep); Foong, J (Foong, Justin); Provart, NJ (Provart, Nicholas J.); Campbell, MM (Campbell, Malcolm M.)

    来源出版物: PLANT PHYSIOLOGY  : 149  : 2  : 981-993  DOI: 10.1104/pp.108.132795  出版年: FEB 2009  

    摘要: The R2R3-MYB proteins comprise one of the largest families of transcription factors in plants. R2R3-MYB family members regulate plant-specific processes, such as the elaboration of specialized cell types, including xylem, guard cells, trichomes, and root hairs, and the biosynthesis of specialized branches of metabolism, including phenylpropanoid biosynthesis. As such, R2R3-MYB family members are hypothesized to contribute to the emergence of evolutionary innovations that have arisen in specific plant lineages. As a first step in determining the role played by R2R3-MYB family members in the emergence of lineage-specific innovations in the genus Populus, the entire Populus trichocarpa R2R3-MYB family was characterized. The Populus R2R3-MYB complement is much larger than that found in other angiosperms with fully sequenced genomes. Phylogenetic analyses, together with chromosome placement, showed that the expansion of the Populus R2R3-MYB family was not only attributable to whole genome duplication but also involved selective expansion of specific R2R3-MYB clades. Expansion of the Populus R2R3-MYB family prominently involved members with expression patterns that suggested a role in specific components of Populus life history, including wood formation and reproductive development. An expandable compendium of microarray-based expression data (PopGenExpress) and associated Web-based tools were developed to better enable within- and between-species comparisons of Populus R2R3-MYB gene expression. This resource, which includes intuitive graphic visualization of gene expression data across multiple tissues, organs, and treatments, is freely available to, and expandable by, scientists wishing to better understand the genome biology of Populus, an ecologically dominant and economically important forest tree genus.

    入藏号: WOS:000263129400033 

    PubMed ID: 19091872 

    ISSN: 0032-0889 

     

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    48 条,共 77

    标题: Pretreatments to enhance the digestibility of lignocellulosic biomass 

    作者: Hendriks, ATWM (Hendriks, A. T. W. M.); Zeeman, G (Zeeman, G.)

    来源出版物: BIORESOURCE TECHNOLOGY  : 100  : 1  : 10-18  DOI: 10.1016/j.biortech.2008.05.027  出版年: JAN 2009  

    摘要: Lignocellulosic biomass represents a rather unused source for biogas and ethanol production. Many factors, like lignin content, crystallinity of cellulose, and particle size, limit the digestibility of the hemicellulose and cellulose present in the lignocellulosic biomass. Pretreatments have as a goal to improve the digestibility of the lignocellulosic biomass. Each pretreatment has its own effect(s) on the cellulose, hemicellulose and lignin; the three main components of lignocellulosic biomass. This paper reviews the different effect(s) of several pretreatments on the three main parts of the lignocellulosic biomass to improve its digestibility. Steam pretreatment, lime pretreatment, liquid hot water pretreatments and ammonia based pretreatments are concluded to be pretreatments with high potentials. The main effects are dissolving hemicellulose and alteration of lignin structure, providing an improved accessibility of the cellulose for hydrolytic enzymes. (c) 2008 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000260152300002 

    PubMed ID: 18599291 

    作者识别号:

    作者 ResearcherID ORCID

    ferreira, filipe  I-5786-2013   

     

    ISSN: 0960-8524 

     

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    49 条,共 77

    标题: Finding and Comparing Syntenic Regions among Arabidopsis and the Outgroups Papaya, Poplar, and Grape: CoGe with Rosids 

    作者: Lyons, E (Lyons, Eric); Pedersen, B (Pedersen, Brent); Kane, J (Kane, Josh); Alam, M (Alam, Maqsudul); Ming, R (Ming, Ray); Tang, HB (Tang, Haibao); Wang, XY (Wang, Xiyin); Bowers, J (Bowers, John); Paterson, A (Paterson, Andrew); Lisch, D (Lisch, Damon); Freeling, M (Freeling, Michael)

    来源出版物: PLANT PHYSIOLOGY  : 148  : 4  : 1772-1781  DOI: 10.1104/pp.108.124867  出版年: DEC 2008  

    摘要: In addition to the genomes of Arabidopsis (Arabidopsis thaliana) and poplar (Populus trichocarpa), two near-complete rosid genome sequences, grape (Vitis vinifera) and papaya (Carica papaya), have been recently released. The phylogenetic relationship among these four genomes and the placement of their three independent, fractionated tetraploidies sum to a powerful comparative genomic system. CoGe, a platform of multiple whole or near-complete genome sequences, provides an integrative Web-based system to find and align syntenic chromosomal regions and visualize the output in an intuitive and interactive manner. CoGe has been customized to specifically support comparisons among the rosids. Crucial facts and definitions are presented to clearly describe the sorts of biological questions that might be answered in part using CoGe, including patterns of DNA conservation, accuracy of annotation, transposability of individual genes, subfunctionalization and/ or fractionation of syntenic gene sets, and conserved noncoding sequence content. This precis of an online tutorial, CoGe with Rosids (http://tinyurl.com/4a23pk), presents sample results graphically.

    入藏号: WOS:000261501500005 

    PubMed ID: 18952863 

    作者识别号:

    作者 ResearcherID ORCID

    Bowers, John  B-9245-2009   

    Lisch, Damon  A-9414-2010   

    Tang, Haibao  A-6715-2011   

    Tang, Haibao    0000-0002-3460-8570 

     

    ISSN: 0032-0889 

     

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    50 条,共 77

    标题: Mechanism study of wood lignin pyrolysis by using TG-FTIR analysis 

    作者: Liu, Q (Liu, Qian); Wang, SR (Wang, Shurong); Zheng, Y (Zheng, Yun); Luo, ZY (Luo, Zhongyang); Cen, KF (Cen, Kefa)

    来源出版物: JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS  : 82  : 1  : 170-177  DOI: 10.1016/j.jaap.2008.03.007  出版年: MAY 2008  

    摘要: In order to study the pyrolysis mechanism of lignin, Van Soest's method was performed to extract lignin from different species of biomass. Fir and birch lignins were selected as the testing samples and corresponding microstructure was studied by a Fourier transform infrared spectrometry. Several characteristic bands were observed to show the presence and species of lignin. Following experimental research on pyrolysis of lignins from fir and birch were carried out on a thermogravimetric analyzer coupled with a Fourier transform infrared spectrometry. Wood lignin undergoes in three consecutive stages, corresponding to the evaporation of water, the formation of primary volatiles and the subsequent release of small molecular gases. The main pyrolysis sections and the maximum weight loss rates are quite different for different wood species. Phenols are the main volatile products, in addition to alcohols, aldehydes, acids, and so on. As the main gaseous products, CO, CO2, CH4 are released out greatly. (C) 2008 Elsevier B.V. All rights reserved.

    入藏号: WOS:000257005900018 

    ISSN: 0165-2370 

     

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    51 条,共 77

    标题: Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models 

    作者: Wiklund, S (Wiklund, Susanne); Johansson, E (Johansson, Erik); Sjostrom, L (Sjostrom, Lina); Mellerowicz, EJ (Mellerowicz, Ewa J.); Edlund, U (Edlund, Ulf); Shockcor, JP (Shockcor, John P.); Gottfries, J (Gottfries, Johan); Moritz, T (Moritz, Thomas); Trygg, J (Trygg, Johan)

    来源出版物: ANALYTICAL CHEMISTRY  : 80  : 1  : 115-122  DOI: 10.1021/ac0713510  出版年: JAN 1 2008  

    摘要: Metabolomics studies generate increasingly complex data tables, which are hard to summarize and visualize without appropriate tools. The use of chemometrics tools, e.g., principal component analysis (PCA), partial least-squares to latent structures (PLS), and orthogonal PLS (OPLS), is therefore of great importance as these include efficient, validated, and robust methods for modeling information-rich chemical and biological data. Here the S-plot is proposed as a tool for visualization and interpretation of multivariate classification models, e.g., OPLS discriminate analysis, having two or more classes. The S-plot visualizes both the covariance and correlation between the metabolites and the modeled class designation. Thereby the S-plot helps identifying statistically significant and potentially biochemically significant metabolites, based both on contributions to the model and their reliability. An extension of the S-plot, the SUS-plot (shared and unique structure), is applied to compare the outcome of multiple classification models compared to a common reference, e.g., control. The used example is a gas chromatography coupled mass spectroscopy based metabolomics study in plant biology where two different transgenic poplar lines are compared to wild type. By using OPLS, an improved visualization and discrimination of interesting metabolites could be demonstrated.

    入藏号: WOS:000252026900021 

    PubMed ID: 18027910 

    ISSN: 0003-2700 

     

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    52 条,共 77

    标题: Isoprene emission from plants: Why and how 

    作者: Sharkey, TD (Sharkey, Thomas D.); Wiberley, AE (Wiberley, Amy E.); Donohue, AR (Donohue, Autumn R.)

    来源出版物: ANNALS OF BOTANY  : 101  : 1  : 5-18  DOI: 10.1093/aob/mcm240  出版年: JAN 2008  

    摘要: Background Some, but not all, plants emit isoprene. Emission of the related monoterpenes is more universal among plants, but the amount of isoprene emitted from plants dominates the biosphere-atmosphere hydrocarbon exchange.

    Scope The emission of isoprene from plants affects atmospheric chemistry. Isoprene reacts very rapidly with hydroxyl radicals in the atmosphere making hydroperoxides that can enhance ozone formation. Aerosol formation in the atmosphere may also be influenced by biogenic isoprene. Plants that emit isoprene are better able to tolerate sunlight-induced rapid heating of leaves (heat flecks). They also tolerate ozone and other reactive oxygen species better than non-emitting plants. Expression of the isoprene synthase gene can account for control of isoprene emission capacity as leaves expand. The emission capacity of fully expanded leaves varies through the season but the biochemical control of capacity of mature leaves appears to be at several different points in isoprene metabolism.

    Conclusions The capacity for isoprene emission evolved many times in plants, probably as a mechanism for coping with heat flecks. It also confers tolerance of reactive oxygen species. It is an example of isoprenoids enhancing membrane function, although the mechanism is likely to be different from that of sterols. Understanding the regulation of isoprene emission is advancing rapidly now that the pathway that provides the substrate is known.

    入藏号: WOS:000251679100002 

    PubMed ID: 17921528 

    作者识别号:

    作者 ResearcherID ORCID

    Sharkey, Thomas  B-4032-2009  0000-0002-4423-3223 

     

    ISSN: 0305-7364 

     

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    53 条,共 77

    标题: The role of glutathione in photosynthetic organisms: Emerging functions for glutaredoxins and glutathionylation 

    作者: Rouhier, N (Rouhier, Nicolas); Lemaire, SD (Lemaire, Stephane D.); Jacquot, JP (Jacquot, Jean-Pierre)

    来源出版物: ANNUAL REVIEW OF PLANT BIOLOGY  丛书: Annual Review of Plant Biology  : 59  : 143-166  DOI: 10.1146/annurev.arplant.59.032607.092811  出版年: 2008   

    摘要: Glutathione, a tripeptide with the sequence gamma-Glu-Cys-Gly, exists either in a reduced form with a free thiol group or in an oxidized form with a disulfide between two identical molecules. We describe here briefly the pathways involved in the synthesis, reduction, polymerization, and degradation of glutathione, as well as its distribution throughout the plant and its redox buffering capacities. The function of glutathione in xenobiotic and heavy metal detoxification, plant development, and plant-pathogen interactions is also briefly discussed. Several lines of evidence indicate that glutathione and glutaredoxins (GRXs) are implicated in the response to oxidative stress through the regeneration of enzymes involved in peroxide and methionine sulfoxide reduction. Finally, emerging functions for plant GRXs and glutathione concern the regulation of protein activity via glutathionylation and the capacity of some GRXs to bind iron sulfur centers and for some of them to transfer FeS clusters into apoproteins.

    入藏号: WOS:000256593200007 

    PubMed ID: 18444899 

    作者识别号:

    作者 ResearcherID ORCID

    Jacquot, Jean-Pierre  A-1571-2012   

    Lemaire, Stephane  A-3530-2008   

    Jacquot, Jean-Pierre    0000-0003-4975-8587 

     

    ISSN: 1543-5008 

    ISBN: 978-0-8243-0659-5

     

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    54 条,共 77

    标题: Enhancing phytoremediation through the use of transgenics and endophytes 

    作者: Doty, SL (Doty, Sharon Lafferty)

    来源出版物: NEW PHYTOLOGIST  : 179  : 2  : 318-333  DOI: 10.1111/j.1469-8137.2008.02446.x  出版年: 2008  

    摘要: In the last decade, there has been an increase in research on improving the ability of plants to remove environmental pollution. Genes from microbes, plants, and animals are being used successfully to enhance the ability of plants to tolerate, remove, and degrade pollutants. Through expression of specific bacterial genes in transgenic plants, the phytotoxic effects of nitroaromatic pollutants were overcome, resulting in increased removal of these chemicals. Overexpression of mammalian genes encoding cytochrome P450s led to increased metabolism and removal of a variety of organic pollutants and herbicides. Genes involved in the uptake or detoxification of metal pollutants were used to enhance phytoremediation of this important class of pollutants. Transgenic plants containing specific bacterial genes converted mercury and selenium to less toxic forms. In addition to these transgenic approaches, the use of microbes that live within plants, termed endophytes, also led to improved tolerance to normally phytotoxic chemicals and increased removal of the pollutants. Bacteria that degraded a herbicide imparted resistance to the herbicide when inoculated into plants. In another study, plants harboring bacteria capable of degrading toluene were more tolerant to normally phytotoxic concentrations of the chemical, and transpired less of it into the atmosphere. This review examines the recent advances in enhancing phytoremediation through transgenic plant research and through the use of symbiotic endophytic microorganisms within plant tissues.

    入藏号: WOS:000257570400006 

    PubMed ID: 19086174 

    ISSN: 0028-646X 

     

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    55 条,共 77

    标题: Downregulation of cinnamoyl-coenzyme a reductase in poplar: Multiple-level phenotyping reveals effects on cell wall polymer metabolism and structure 

    作者: Leple, JC (Leple, Jean-Charles); Dauwe, R (Dauwe, Rebecca); Morreel, K (Morreel, Kris); Storme, V (Storme, Veronique); Lapierre, C (Lapierre, Catherine); Pollet, B (Pollet, Brigitte); Naumann, A (Naumann, Annette); Kang, KY (Kang, Kyu-Young); Kim, H (Kim, Hoon); Ruel, K (Ruel, Katia); Lefebvre, A (Lefebvre, Andree); Joseleau, JP (Joseleau, Jean-Paul); Grima-Pettenati, J (Grima-Pettenati, Jacqueline); De Rycke, R (De Rycke, Riet); Andersson-Gunneras, S (Andersson-Gunneras, Sara); Erban, A (Erban, Alexander); Fehrle, I (Fehrle, Ines); Petit-Conil, M (Petit-Conil, Michel); Kopka, J (Kopka, Joachim); Polle, A (Polle, Andrea); Messens, E (Messens, Eric); Sundberg, B (Sundberg, Bjorn); Mansfield, SD (Mansfield, Shawn D.); Ralph, J (Ralph, John); Pilate, G (Pilate, Gilles); Boerjan, W (Boerjan, Wout)

    来源出版物: PLANT CELL  : 19  : 11  : 3669-3691  DOI: 10.1105/tpc.107.054148  出版年: NOV 2007  

    摘要: Cinnamoyl-CoA reductase (CCR) catalyzes the penultimate step in monolignol biosynthesis. We show that downregulation of CCR in transgenic poplar (Populus tremula 3 Populus alba) was associated with up to 50% reduced lignin content and an orange-brown, often patchy, coloration of the outer xylem. Thioacidolysis, nuclear magnetic resonance (NMR), immunocytochemistry of lignin epitopes, and oligolignol profiling indicated that lignin was relatively more reduced in syringyl than in guaiacyl units. The cohesion of the walls was affected, particularly at sites that are generally richer in syringyl units in wild-type poplar. Ferulic acid was incorporated into the lignin via ether bonds, as evidenced independently by thioacidolysis and by NMR. A synthetic lignin incorporating ferulic acid had a red-brown coloration, suggesting that the xylem coloration was due to the presence of ferulic acid during lignification. Elevated ferulic acid levels were also observed in the form of esters. Transcript and metabolite profiling were used as comprehensive phenotyping tools to investigate how CCR downregulation impacted metabolism and the biosynthesis of other cell wall polymers. Both methods suggested reduced biosynthesis and increased breakdown or remodeling of noncellulosic cell wall polymers, which was further supported by Fourier transform infrared spectroscopy and wet chemistry analysis. The reduced levels of lignin and hemicellulose were associated with an increased proportion of cellulose. Furthermore, the transcript and metabolite profiling data pointed toward a stress response induced by the altered cell wall structure. Finally, chemical pulping of wood derived from 5-year-old, field-grown transgenic lines revealed improved pulping characteristics, but growth was affected in all transgenic lines tested.

    入藏号: WOS:000252268700027 

    PubMed ID: 18024569 

    作者识别号:

    作者 ResearcherID ORCID

    Pilate, Gilles  D-1666-2011   

    LEPLE, Jean-Charles  H-4210-2012   

    LEPLE, Jean charles  L-9604-2014   

    Ralph, John  C-3161-2009   

     

    ISSN: 1040-4651 

     

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    56 条,共 77

    标题: A molecular timetable for apical bud formation and dormancy induction in poplar 

    作者: Ruttink, T (Ruttink, Tom); Arend, M (Arend, Matthias); Morreel, K (Morreel, Kris); Storme, V (Storme, Veronique); Rombauts, S (Rombauts, Stephane); Fromm, J (Fromm, Joerg); Bhalerao, RP (Bhalerao, Rishikesh P.); Boerjan, W (Boerjan, Wout); Rohde, A (Rohde, Antje)

    来源出版物: PLANT CELL  : 19  : 8  : 2370-2390  DOI: 10.1105/tpc.107.052811  出版年: AUG 2007  

    摘要: The growth of perennial plants in the temperate zone alternates with periods of dormancy that are typically initiated during bud development in autumn. In a systems biology approach to unravel the underlying molecular program of apical bud development in poplar (Populus tremula 3 Populus alba), combined transcript and metabolite profiling were applied to a high-resolution time course from short-day induction to complete dormancy. Metabolite and gene expression dynamics were used to reconstruct the temporal sequence of events during bud development. Importantly, bud development could be dissected into bud formation, acclimation to dehydration and cold, and dormancy. To each of these processes, specific sets of regulatory and marker genes and metabolites are associated and provide a reference frame for future functional studies. Light, ethylene, and abscisic acid signal transduction pathways consecutively control bud development by setting, modifying, or terminating these processes. Ethylene signal transduction is positioned temporally between light and abscisic acid signals and is putatively activated by transiently low hexose pools. The timing and place of cell proliferation arrest (related to dormancy) and of the accumulation of storage compounds (related to acclimation processes) were established within the bud by electron microscopy. Finally, the identification of a large set of genes commonly expressed during the growth-to-dormancy transitions in poplar apical buds, cambium, or Arabidopsis thaliana seeds suggests parallels in the underlying molecular mechanisms in different plant organs.

    入藏号: WOS:000249873800006 

    PubMed ID: 17693531 

    作者识别号:

    作者 ResearcherID ORCID

    Arend, Matthias  L-7795-2013   

    Rombauts, Stephane  D-7640-2014  0000-0002-3985-4981 

     

    ISSN: 1040-4651 

     

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    57 条,共 77

    标题: PlnTFDB: an integrative plant transcription factor database 

    作者: Riano-Pachon, DM (Riano-Pachon, Diego Mauricio); Ruzicic, S (Ruzicic, Slobodan); Dreyer, I (Dreyer, Ingo); Mueller-Roeber, B (Mueller-Roeber, Bernd)

    来源出版物: BMC BIOINFORMATICS  : 8  文献号: 42  DOI: 10.1186/1471-2105-8-42  出版年: FEB 7 2007  

    摘要: Background: Transcription factors (TFs) are key regulatory proteins that enhance or repress the transcriptional rate of their target genes by binding to specific promoter regions (i.e. cis-acting elements) upon activation or de-activation of upstream signaling cascades. TFs thus constitute master control elements of dynamic transcriptional networks. TFs have fundamental roles in almost all biological processes ( development, growth and response to environmental factors) and it is assumed that they play immensely important functions in the evolution of species. In plants, TFs have been employed to manipulate various types of metabolic, developmental and stress response pathways. Cross-species comparison and identification of regulatory modules and hence TFs is thought to become increasingly important for the rational design of new plant biomass. Up to now, however, no computational repository is available that provides access to the largely complete sets of transcription factors of sequenced plant genomes.

    Description: PlnTFDB is an integrative plant transcription factor database that provides a web interface to access large ( close to complete) sets of transcription factors of several plant species, currently encompassing Arabidopsis thaliana (thale cress), Populus trichocarpa ( poplar), Oryza sativa ( rice), Chlamydomonas reinhardtii and Ostreococcus tauri. It also provides an access point to its daughter databases of a species-centered representation of transcription factors (OstreoTFDB, ChlamyTFDB, ArabTFDB, PoplarTFDB and RiceTFDB). Information including protein sequences, coding regions, genomic sequences, expressed sequence tags ( ESTs), domain architecture and scientific literature is provided for each family.

    Conclusion: We have created lists of putatively complete sets of transcription factors and other transcriptional regulators for five plant genomes. They are publicly available through http://plntfdb.bio.uni-potsdam.de. Further data will be included in the future when the sequences of other plant genomes become available.

    入藏号: WOS:000244469500001 

    PubMed ID: 17286856 

    作者识别号:

    作者 ResearcherID ORCID

    Riano-Pachon, Diego  A-1755-2010  0000-0001-9803-3465 

    Dreyer, Ingo  N-4747-2014   

     

    ISSN: 1471-2105 

     

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    58 条,共 77

    标题: Arabidopsis irregular xylem8 and irregular xylem9: Implications for the complexity of glucuronoxylan biosynthesis 

    作者: Pena, MJ (Pena, Maria J.); Zhong, RQ (Zhong, Ruiqin); Zhou, GK (Zhou, Gong-Ke); Richardson, EA (Richardson, Elizabeth A.); O'Neill, MA (O'Neill, Malcolm A.); Darvill, AG (Darvill, Alan G.); York, WS (York, William S.); Ye, ZH (Ye, Zheng-Hua)

    来源出版物: PLANT CELL  : 19  : 2  : 549-563  DOI: 10.1105/tpc.106.049320  出版年: FEB 2007  

    摘要: Mutations of Arabidopsis thaliana IRREGULAR XYLEM8 (IRX8) and IRX9 were previously shown to cause a collapsed xylem phenotype and decreases in xylose and cellulose in cell walls. In this study, we characterized IRX8 and IRX9 and performed chemical and structural analyses of glucuronoxylan (GX) from irx8 and irx9 plants. IRX8 and IRX9 are expressed specifically in cells undergoing secondary wall thickening, and their encoded proteins are targeted to the Golgi, where GX is synthesized. 1H-NMR spectroscopy showed that the reducing end of Arabidopsis GX contains the glycosyl sequence 4-beta-D-Xylp(1 -> 4)-beta-D-Xylp-(1 -> 3)-alpha-L-Rhap-(1 -> 2)-alpha-D-GalpA-(1 -> 4)- D-Xylp, which was previously identified in birch ( Betula verrucosa) and spruce ( Picea abies) GX. This indicates that the reducing end structure of GXs is evolutionarily conserved in woody and herbaceous plants. This sequence is more abundant in irx9 GX than in the wild type, whereas irx8 and fragile fiber8 (fra8) plants are nearly devoid of it. The number of GX chains increased and the GX chain length decreased in irx9 plants. Conversely, the number of GX chains decreased and the chain length heterodispersity increased in irx8 and fra8 plants. Our results suggest that IRX9 is required for normal GX elongation and indicate roles for IRX8 and FRA8 in the synthesis of the glycosyl sequence at the GX reducing end.

    入藏号: WOS:000245467700017 

    PubMed ID: 17322407 

    ISSN: 1040-4651 

     

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    59 条,共 77

    标题: Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology, and growth performance in Populus euphratica, a poplar growing in arid regions 

    作者: Bogeat-Triboulot, MB (Bogeat-Triboulot, Marie-Beatrice); Brosche, M (Brosche, Mikael); Renaut, J (Renaut, Jenny); Jouve, L (Jouve, Laurent); Le Thiec, D (Le Thiec, Didier); Fayyaz, P (Fayyaz, Payam); Vinocur, B (Vinocur, Basia); Witters, E (Witters, Erwin); Laukens, K (Laukens, Kris); Teichmann, T (Teichmann, Thomas); Altman, A (Altman, Arie); Hausman, JF (Hausman, Jean-Francois); Polle, A (Polle, Andrea); Kangasjarvi, J (Kangasjarvi, Jaakko); Dreyer, E (Dreyer, Erwin)

    来源出版物: PLANT PHYSIOLOGY  : 143  : 2  : 876-892  DOI: 10.1104/pp.106.088708  出版年: FEB 2007   

    摘要: The responses of Populus euphratica Oliv. plants to soil water deficit were assessed by analyzing gene expression, protein profiles, and several plant performance criteria to understand the acclimation of plants to soil water deficit. Young, vegetatively propagated plants originating from an arid, saline field site were submitted to a gradually increasing water deficit for 4 weeks in a greenhouse and were allowed to recover for 10 d after full reirrigation. Time-dependent changes and intensity of the perturbations induced in shoot and root growth, xylem anatomy, gas exchange, and water status were recorded. The expression profiles of approximately 6,340 genes and of proteins and metabolites (pigments, soluble carbohydrates, and oxidative compounds) were also recorded in mature leaves and in roots (gene expression only) at four stress levels and after recovery. Drought successively induced shoot growth cessation, stomatal closure, moderate increases in oxidative stress-related compounds, loss of CO2 assimilation, and root growth reduction. These effects were almost fully reversible, indicating that acclimation was dominant over injury. The physiological responses were paralleled by fully reversible transcriptional changes, including only 1.5% of the genes on the array. Protein profiles displayed greater changes than transcript levels. Among the identified proteins for which expressed sequence tags were present on the array, no correlation was found between transcript and protein abundance. Acclimation to water deficit involves the regulation of different networks of genes in roots and shoots. Such diverse requirements for protecting and maintaining the function of different plant organs may render plant engineering or breeding toward improved drought tolerance more complex than previously anticipated.

    入藏号: WOS:000244032400029 

    PubMed ID: 17158588 

    作者识别号:

    作者 ResearcherID ORCID

    Didier, LE THIEC  C-7100-2008   

    renaut, jenny  K-3216-2012  0000-0002-0450-3866 

    Didier, Le Thiec  C-9531-2013   

    Laukens, Kris  B-7806-2012  0000-0002-8217-2564 

    HAUSMAN, Jean-Francois  D-1356-2009  0000-0001-6103-7817 

    Kangasjarvi, Jaakko  A-3024-2015   

    Brosche, Mikael  H-2260-2015  0000-0002-1135-2496 

     

    ISSN: 0032-0889 

     

    --------------------------------------------------------------------------------

    60 条,共 77

    标题: Populus: A model system for plant biology 

    作者: Jansson, S (Jansson, Stefan); Douglas, CJ (Douglas, Carl J.)

    来源出版物: ANNUAL REVIEW OF PLANT BIOLOGY  丛书: Annual Review of Plant Biology  : 58  : 435-458  DOI: 10.1146/annurev.arplant.58.032806.103956  出版年: 2007  

    摘要: With the completion of the Populus trichocarpa genome sequence and the development of various genetic, genomic, and biochemical tools, Populus now offers many possibilities to study questions that cannot be as easily addressed in Arabidopsis and rice, the two prime model systems of plant biology and genomics. Tree-specific traits such as wood formation, long-term perennial growth, and seasonality are obvious areas of research, but research in other areas such as control of flowering, biotic interactions, and evolution of adaptive traits is enriched by adding a tree to the suite of model systems. Furthermore, the reproductive biology of Populus (a dioeceous wind-pollinated long-lived tree) offers both new possibilities and challenges in the study and analysis of natural genetic and phenotypic variation. The relatively close phylogenetic relationship of Populus to Arabidopsis in the Eurosid clade of Eudicotyledonous plants aids in comparative functional studies and comparative genomics, and has the potential to greatly facilitate studies on genome and gene family evolution in eudicots.

    入藏号: WOS:000247703600020 

    PubMed ID: 17280524 

    作者识别号:

    作者 ResearcherID ORCID

    Jansson, Stefan  A-1119-2009  0000-0002-7906-6891 

    Douglas, Carl  B-1384-2013   

     

    ISSN: 1543-5008 

    ISBN: 978-0-8243-0658-8

     

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    61 条,共 77

    标题: Mechanisms controlling soil carbon turnover and their potential application for enhancing carbon sequestration 

    作者: Jastrow, JD (Jastrow, Julie D.); Amonette, JE (Amonette, James E.); Bailey, VL (Bailey, Vanessa L.)

    来源出版物: CLIMATIC CHANGE  : 80  : 1-2  : 5-23  DOI: 10.1007/s10584-006-9178-3  出版年: JAN 2007  

    摘要: In addition to increasing plant C inputs, strategies for enhancing soil C sequestration include reducing C turnover and increasing its residence time in soils. Two major mechanisms, (bio)chemical alteration and physicochemical protection, stabilize soil organic C (SOC) and thereby control its turnover. With (bio)chemical alteration, SOC is transformed by biotic and abiotic processes to chemical forms that are more resistant to decomposition and, in some cases, more easily retained by sorption to soil solids. With physicochemical protection, biochemical attack of SOC is inhibited by organomineral interactions at molecular to millimeter scales. Stabilization of otherwise decomposable SOC can occur via sorption to mineral and organic soil surfaces, occlusion within aggregates, and deposition in pores or other locations inaccessible to decomposers and extracellular enzymes. Soil structure is a master integrating variable that both controls and indicates the SOC stabilization status of a soil. One potential option for reducing SOC turnover and enhancing sequestration, is to modify the soil physicochemical environment to favor the activities of fungi. Specific practices that could accomplish this include manipulating the quality of plant C inputs, planting perennial species, minimizing tillage and other disturbances, maintaining a near-neutral soil pH and adequate amounts of exchangeable base cations (particularly calcium), ensuring adequate drainage, and minimizing erosion. In some soils, amendment with micro- and mesoporous sorbents that have a high specific surface - such as fly ash or charcoal - can be beneficial.

    入藏号: WOS:000243555300002 

    作者识别号:

    作者 ResearcherID ORCID

    daorui, han  G-3767-2011   

     

    ISSN: 0165-0009 

     

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    62 条,共 77

    标题: NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis 

    作者: Mitsuda, N (Mitsuda, Nobutaka); Iwase, A (Iwase, Akira); Yamamoto, H (Yamamoto, Hiroyuki); Yoshida, M (Yoshida, Masato); Seki, M (Seki, Motoaki); Shinozaki, K (Shinozaki, Kazuo); Ohme-Takagi, M (Ohme-Takagi, Masaru)

    来源出版物: PLANT CELL  : 19  : 1  : 270-280  DOI: 10.1105/tpc.106.047043  出版年: JAN 2007  

    摘要: Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of lignin and cellulose. Several genes involved in lignin and cellulose biosynthesis have been characterized, but the factors that regulate the formation of secondary walls in woody tissues remain to be identified. In this study, we show that plant-specific transcription factors, designated NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis thaliana. In nst1-1 nst3-1 double knockout plants, the secondary wall thickenings in interfascicular fibers and secondary xylem, except for vascular vessels, were completely suppressed without affecting formation of cells destined to be woody tissues. Conversely, as shown previously for NST1, overexpression of NST3 induced ectopic secondary wall thickenings in various aboveground tissues. Furthermore, the expression of chimeric repressors derived from NST1 and NST3 suppressed secondary wall thickenings in the presumptive interfascicular fibers. Because putative orthologs of NST1 and NST3 are present in the genome of poplar, our results suggest that they are also key regulators of the formation of secondary walls in woody plants and could be used as a tool for the genetic engineering of wood and its derivatives.

    入藏号: WOS:000244757400023 

    PubMed ID: 17237351 

    作者识别号:

    作者 ResearcherID ORCID

    Shinozaki, Kazuo  G-4202-2013   

    Seki, Motoaki  I-2854-2012   

     

    ISSN: 1040-4651 

     

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    63 条,共 77

    标题: Cadmium in plants on polluted soils: Effects of soil factors, hyperaccumulation, and amendments 

    作者: Kirkham, MB (Kirkham, M. B.)

    来源出版物: GEODERMA  : 137  : 1-2  : 19-32  DOI: 10.1016/j.geoderma.2006.08.024  出版年: DEC 31 2006  

    摘要: Cadmium (Cd) is a heavy metal that is of great concern in the environment, because of its toxicity to animals and humans. This article reviews recent papers showing how soil factors (such as pH, phosphate, zinc, and organic matter), Cd hyperaccumulation, and soil amendments affect Cd availability. The studies confirm that the pH of the soil is usually the most important factor that controls uptake, with low pH favoring Cd accumulation, and that phosphate and zinc decrease Cd uptake. The work reveals that the availability of Cd is increased by the application of chloride and reduced by application of silicon. The most striking result of this review is the elevated levels of Cd in plants that are being reported in recent studies. Data for concentrations of Cd in soils and plants under variously polluted conditions are presented in a table and show that all plants have Cd concentrations >= 0.1 mg/kg, the normal concentration in plants. Concentrations ranged from two low concentrations of 0.1 mg/kg Cd (in grain of corn, Zea mays, on an abandoned sludge disposal site that had not received sludge for 10 years, and in roots of hybrid poplar, Populus deltoides x P. nigra, at a 25-year old active sludge farm) to 380 mg/kg Cd in leaves of penny-cress (Thlaspi caerulescens). Plants that hyperaccumulate Cd (i.e., have 100 mg/kg Cd in the tissue or more) belong to the genus Thalspi, the only known Cd hyperaccumulator. Of particular concern for humans are the high concentrations of Cd in rice grain and tobacco leaves, Even if Cd availability is decreased by adding amendments, it is still in the soil and a potential hazard. The best solution for maintaining non-contaminated soils and plants is to remove the sources of Cd in the environment. Given that that is essentially impossible at this time, further research needs to determine how soil and plant factors affect Cd availability on polluted soils. (c) 2006 Elsevier B.V All tights reserved.

    入藏号: WOS:000243601000003 

    ISSN: 0016-7061 

     

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    64 条,共 77

    标题: Polyphenol oxidases in plants and fungi: Going places? A review 

    作者: Mayer, AM (Mayer, Alfred M.)

    来源出版物: PHYTOCHEMISTRY  : 67  : 21  : 2318-2331  DOI: 10.1016/j.phytochem.2006.08.006  出版年: NOV 2006  

    摘要: The more recent reports on polyphenol oxidase in plants and fungi are reviewed. The main aspects considered are the structure, distribution, location and properties of polyphenol oxidase (PPO) as well as newly discovered inhibitors of the enzyme. Particular stress is given to the possible function of the enzyme. The cloning and characterization of a large number of PPOs is surveyed. Although the active site of the enzyme is conserved, the amino acid sequence shows very considerable variability among species. Most plants and fungi PPO have multiple forms of PPO. Expression of the genes coding for the enzyme is tissue specific and also developmentally controlled. Many inhibitors of PPO have been described, which belong to very diverse chemical structures; however, their usefulness for controlling PPO activity remains in doubt. The function of PPO still remains enigmatic. In plants the positive correlation between levels of PPO and the resistance to pathogens and herbivores is frequently observed, but convincing proof of a causal relationship, in most cases, still has not been published. Evidence for the induction of PPO in plants, particularly under conditions of stress and pathogen attack is considered, including the role of jasmonate in the induction process. A clear role of PPO in a least two biosynthetic processes has been clearly demonstrated. In both cases a very high degree of substrate specificity has been found. In fungi, the function of PPO is probably different from that in plants, but there is some evidence indicating that here too PPO has a role in defense against pathogens. PPO also may be a pathogenic factor during the attack of fungi on other organisms. Although many details about structure and probably function of PPO have been revealed in the period reviewed, some of the basic questions raised over the years remain to be answered. (c) 2006 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000241931300002 

    PubMed ID: 16973188 

    ISSN: 0031-9422 

     

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    65 条,共 77

    标题: The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) 

    作者: Tuskan, GA (Tuskan, G. A.); DiFazio, S (DiFazio, S.); Jansson, S (Jansson, S.); Bohlmann, J (Bohlmann, J.); Grigoriev, I (Grigoriev, I.); Hellsten, U (Hellsten, U.); Putnam, N (Putnam, N.); Ralph, S (Ralph, S.); Rombauts, S (Rombauts, S.); Salamov, A (Salamov, A.); Schein, J (Schein, J.); Sterck, L (Sterck, L.); Aerts, A (Aerts, A.); Bhalerao, RR (Bhalerao, R. R.); Bhalerao, RP (Bhalerao, R. P.); Blaudez, D (Blaudez, D.); Boerjan, W (Boerjan, W.); Brun, A (Brun, A.); Brunner, A (Brunner, A.); Busov, V (Busov, V.); Campbell, M (Campbell, M.); Carlson, J (Carlson, J.); Chalot, M (Chalot, M.); Chapman, J (Chapman, J.); Chen, GL (Chen, G. -L.); Cooper, D (Cooper, D.); Coutinho, PM (Coutinho, P. M.); Couturier, J (Couturier, J.); Covert, S (Covert, S.); Cronk, Q (Cronk, Q.); Cunningham, R (Cunningham, R.); Davis, J (Davis, J.); Degroeve, S (Degroeve, S.); Dejardin, A (Dejardin, A.); Depamphilis, C (dePamphilis, C.); Detter, J (Detter, J.); Dirks, B (Dirks, B.); Dubchak, I (Dubchak, I.); Duplessis, S (Duplessis, S.); Ehlting, J (Ehlting, J.); Ellis, B (Ellis, B.); Gendler, K (Gendler, K.); Goodstein, D (Goodstein, D.); Gribskov, M (Gribskov, M.); Grimwood, J (Grimwood, J.); Groover, A (Groover, A.); Gunter, L (Gunter, L.); Hamberger, B (Hamberger, B.); Heinze, B (Heinze, B.); Helariutta, Y (Helariutta, Y.); Henrissat, B (Henrissat, B.); Holligan, D (Holligan, D.); Holt, R (Holt, R.); Huang, W (Huang, W.); Islam-Faridi, N (Islam-Faridi, N.); Jones, S (Jones, S.); Jones-Rhoades, M (Jones-Rhoades, M.); Jorgensen, R (Jorgensen, R.); Joshi, C (Joshi, C.); Kangasjarvi, J (Kangasjarvi, J.); Karlsson, J (Karlsson, J.); Kelleher, C (Kelleher, C.); Kirkpatrick, R (Kirkpatrick, R.); Kirst, M (Kirst, M.); Kohler, A (Kohler, A.); Kalluri, U (Kalluri, U.); Larimer, F (Larimer, F.); Leebens-Mack, J (Leebens-Mack, J.); Leple, JC (Leple, J. -C.); Locascio, P (Locascio, P.); Lou, Y (Lou, Y.); Lucas, S (Lucas, S.); Martin, F (Martin, F.); Montanini, B (Montanini, B.); Napoli, C (Napoli, C.); Nelson, DR (Nelson, D. R.); Nelson, C (Nelson, C.); Nieminen, K (Nieminen, K.); Nilsson, O (Nilsson, O.); Pereda, V (Pereda, V.); Peter, G (Peter, G.); Philippe, R (Philippe, R.); Pilate, G (Pilate, G.); Poliakov, A (Poliakov, A.); Razumovskaya, J (Razumovskaya, J.); Richardson, P (Richardson, P.); Rinaldi, C (Rinaldi, C.); Ritland, K (Ritland, K.); Rouze, P (Rouze, P.); Ryaboy, D (Ryaboy, D.); Schmutz, J (Schmutz, J.); Schrader, J (Schrader, J.); Segerman, B (Segerman, B.); Shin, H (Shin, H.); Siddiqui, A (Siddiqui, A.); Sterky, F (Sterky, F.); Terry, A (Terry, A.); Tsai, CJ (Tsai, C. -J.); Uberbacher, E (Uberbacher, E.); Unneberg, P (Unneberg, P.); Vahala, J (Vahala, J.); Wall, K (Wall, K.); Wessler, S (Wessler, S.); Yang, G (Yang, G.); Yin, T (Yin, T.); Douglas, C (Douglas, C.); Marra, M (Marra, M.); Sandberg, G (Sandberg, G.); Van de Peer, Y (Van de Peer, Y.); Rokhsar, D (Rokhsar, D.)

    来源出版物: SCIENCE  : 313  : 5793  : 1596-1604  DOI: 10.1126/science.1128691  出版年: SEP 15 2006  

    摘要: We report the draft genome of the black cottonwood tree, Populus trichocarpa. Integration of shotgun sequence assembly with genetic mapping enabled chromosome-scale reconstruction of the genome. More than 45,000 putative protein-coding genes were identified. Analysis of the assembled genome revealed a whole-genome duplication event; about 8000 pairs of duplicated genes from that event survived in the Populus genome. A second, older duplication event is indistinguishably coincident with the divergence of the Populus and Arabidopsis lineages. Nucleotide substitution, tandem gene duplication, and gross chromosomal rearrangement appear to proceed substantially more slowly in Populus than in Arabidopsis. Populus has more protein-coding genes than Arabidopsis, ranging on average from 1.4 to 1.6 putative Populus homologs for each Arabidopsis gene. However, the relative frequency of protein domains in the two genomes is similar. Overrepresented exceptions in Populus include genes associated with lignocellulosic wall biosynthesis, meristem development, disease resistance, and metabolite transport.

    入藏号: WOS:000240498900035 

    PubMed ID: 16973872 

    作者识别号:

    作者 ResearcherID ORCID

    Putnam, Nicholas  B-9968-2008  0000-0002-1315-782X 

    Coutinho, Pedro  C-4473-2008   

    Tsai, CJ  C-2450-2009  0000-0002-9282-7704 

    Pereda-Loth, Veronica  A-1296-2011   

    KALLURI, UDAYA  A-6218-2011   

    Tuskan, Gerald  A-6225-2011   

    Van de Peer, Yves  D-4388-2009  0000-0003-4327-3730 

    Pilate, Gilles  D-1666-2011   

    DUPLESSIS, SEBASTIEN  G-4150-2011   

    Jansson, Stefan  A-1119-2009  0000-0002-7906-6891 

    LEPLE, Jean-Charles  H-4210-2012   

    Holt, Robert  C-3303-2009   

    Henrissat, Bernard  J-2475-2012   

    Hamberger, Bjoern  I-2122-2012  0000-0003-1249-1807 

    Douglas, Carl  B-1384-2013   

    Marra, Marco  B-5987-2008   

    Heinze, Berthold  A-8734-2011  0000-0002-7571-9138 

    Schmutz, Jeremy  N-3173-2013   

    Tang, Macy  B-9798-2014   

    Rombauts, Stephane  D-7640-2014  0000-0002-3985-4981 

    CHALOT, Michel  A-1113-2012   

    LEPLE, Jean charles  L-9604-2014   

    Kangasjarvi, Jaakko  A-3024-2015   

    Karlsson, Jan  E-7199-2015  0000-0002-6002-929X 

    Jorgensen, Richard    0000-0002-0382-2371 

    Nelson, David    0000-0003-0583-5421 

    Gribskov, Michael    0000-0002-1718-0242 

     

    ISSN: 0036-8075 

     

    --------------------------------------------------------------------------------

    66 条,共 77

    标题: Organosolv ethanol lignin from hybrid poplar as a radical scavenger: Relationship between lignin structure, extraction conditions, and antioxidant activity 

    作者: Pan, XJ (Pan, Xuejun); Kadla, JF (Kadla, John F.); Ehara, K (Ehara, Katsunobu); Gilkes, N (Gilkes, Neil); Saddler, JN (Saddler, Jack N.)

    来源出版物: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY  : 54  : 16  : 5806-5813  DOI: 10.1021/jf0605392  出版年: AUG 9 2006  

    摘要: Twenty-one organosolv ethanol lignin samples were prepared from hybrid poplar (Populus nigra x P. maximowiczii) under varied conditions with an experimental matrix designed using response surface methodology (RSM). The lignin preparations were evaluated as potential antioxidants. Results indicated that the lignins with more phenolic hydroxyl groups, less aliphatic hydroxyl groups, low molecular weight, and narrow polydispersity showed high antioxidant activity. Processing conditions affected the functional groups and molecular weight of the extracted organosolv ethanol lignins, and consequently influenced the antioxidant activity of the lignins. In general, the lignins prepared at elevated temperature, longer reaction time, increased catalyst, and diluted ethanol showed high antioxidant activity. Regression models were developed to enable the quantitative prediction of lignin characteristics and antioxidant activity based on the processing conditions.

    入藏号: WOS:000239454700015 

    PubMed ID: 16881681 

    作者识别号:

    作者 ResearcherID ORCID

    Saddler, Jack (John)  A-9103-2013   

     

    ISSN: 0021-8561 

     

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    67 条,共 77

    标题: Poplar FT2 shortens the juvenile phase and promotes seasonal flowering 

    作者: Hsu, CY (Hsu, Chuan-Yu); Liu, YX (Liu, Yunxia); Luthe, DS (Luthe, Dawn S.); Yuceer, C (Yuceer, Cetin)

    来源出版物: PLANT CELL  : 18  : 8  : 1846-1861  DOI: 10.1105/tpc.106.041038  出版年: AUG 2006  

    摘要: Many woody perennials, such as poplar (Populus deltoides), are not able to form flower buds during the first several years of their life cycle. They must undergo a transition from the juvenile phase to the reproductive phase to be competent to produce flower buds. After this transition, trees begin to form flower buds in the spring of each growing season. The genetic factors that control flower initiation, ending the juvenile phase, are unknown in poplar. The factors that regulate seasonal flower bud formation are also unknown. Here, we report that poplar FLOWERING LOCUS T2 (FT2), a relative of the Arabidopsis thaliana flowering-time gene FT, controls first-time and seasonal flowering in poplar. The FT2 transcript is rare during the juvenile phase of poplar. When juvenile poplar is transformed with FT2 and transcript levels are increased, flowering is induced within 1 year. During the transition between vegetative and reproductive growth in mature trees, FT2 transcripts are abundant during reproductive growth under long days. Subsequently, floral meristems emerge on flanks of the axillary inflorescence shoots. These findings suggest that FT2 is part of the flower initiation pathway in poplar and plays an additional role in regulating seasonal flower initiation that is integrated with the poplar perennial growth habit.

    入藏号: WOS:000239703000007 

    PubMed ID: 16844908 

    ISSN: 1040-4651 

     

    --------------------------------------------------------------------------------

    68 条,共 77

    标题: Ancient signals: comparative genomics of plant MAPK and MAPKK gene families 

    作者: Hamel, LP (Hamel, Louis-Philippe); Nicole, MC (Nicole, Marie-Claude); Sritubtim, S (Sritubtim, Somrudee); Morency, MJ (Morency, Marie-Jose); Ellis, M (Ellis, Margaret); Ehlting, J (Ehlting, Juergen); Beaudoin, N (Beaudoin, Nathalie); Barbazuk, B (Barbazuk, Brad); Klessig, D (Klessig, Dan); Lee, J (Lee, Justin); Martin, G (Martin, Greg); Mundy, J (Mundy, John); Ohashi, Y (Ohashi, Yuko); Scheel, D (Scheel, Dierk); Sheen, J (Sheen, Jen); Xing, T (Xing, Tim); Zhang, SQ (Zhang, Shuqun); Seguin, A (Seguin, Armand); Ellis, BE (Ellis, Brian E.)

    来源出版物: TRENDS IN PLANT SCIENCE  : 11  : 4  : 192-198  DOI: 10.1016/j.tplants.2006.02.007  出版年: APR 2006  

    摘要: MAPK signal transduction modules play crucial roles in regulating many biological processes in plants, and their components are encoded by highly conserved genes. The recent availability of genome sequences for rice and poplar now makes it possible to examine how well the previously described Arabidopsis MAPK and MAPKK gene family structures represent the broader evolutionary situation in plants, and analysis of gene expression data for MPK and MKK genes in all three species allows further refinement of those families, based on functionality. The Arabidopsis MAPK nomenclature appears sufficiently robust to allow it to be usefully extended to other well-characterized plant systems.

    入藏号: WOS:000237756400007 

    PubMed ID: 16537113 

    作者识别号:

    作者 ResearcherID ORCID

    Martin, Gregory  F-6262-2011  0000-0003-0044-6830 

    Lee, Justin  B-6096-2012  0000-0001-8269-7494 

    mundy, john  A-2361-2013  0000-0001-7490-4588 

     

    ISSN: 1360-1385 

     

    --------------------------------------------------------------------------------

    69 条,共 77

    标题: Poplar carbohydrate-active enzymes. Gene identification and expression analyses 

    作者: Geisler-Lee, J (Geisler-Lee, J); Geisler, M (Geisler, M); Coutinho, PM (Coutinho, PM); Segerman, B (Segerman, B); Nishikubo, N (Nishikubo, N); Takahashi, J (Takahashi, J); Aspeborg, H (Aspeborg, H); Djerbi, S (Djerbi, S); Master, E (Master, E); Andersson-Gunneras, S (Andersson-Gunneras, S); Sundberg, B (Sundberg, B); Karpinski, S (Karpinski, S); Teeri, TT (Teeri, TT); Kleczkowski, LA (Kleczkowski, LA); Henrissat, B (Henrissat, B); Mellerowicz, EJ (Mellerowicz, EJ)

    来源出版物: PLANT PHYSIOLOGY  : 140  : 3  : 946-962  DOI: 10.1104/pp.105.072652  出版年: MAR 2006  

    摘要: Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr.&Gray) genome were identified based on sequence homology, annotated, and grouped into families of glycosyltransferases, glycoside hydrolases, carbohydrate esterases, polysaccharide lyases, and expansins. Poplar ( Populus spp.) had approximately 1.6 times more CAZyme genes than Arabidopsis ( Arabidopsis thaliana). Whereas most families were proportionally increased, xylan and pectin-related families were underrepresented and the GT1 family of secondary metabolite-glycosylating enzymes was overrepresented in poplar. CAZyme gene expression in poplar was analyzed using a collection of 100,000 expressed sequence tags from 17 different tissues and compared to microarray data for poplar and Arabidopsis. Expression of genes involved in pectin and hemicellulose metabolism was detected in all tissues, indicating a constant maintenance of transcripts encoding enzymes remodeling the cell wall matrix. The most abundant transcripts encoded sucrose synthases that were specifically expressed in wood-forming tissues along with cellulose synthase and homologs of KORRIGAN and ELP1. Woody tissues were the richest source of various other CAZyme transcripts, demonstrating the importance of this group of enzymes for xylogenesis. In contrast, there was little expression of genes related to starch metabolism during wood formation, consistent with the preferential flux of carbon to cell wall biosynthesis. Seasonally dormant meristems of poplar showed a high prevalence of transcripts related to starch metabolism and surprisingly retained transcripts of some cell wall synthesis enzymes. The data showed profound changes in CAZyme transcriptomes in different poplar tissues and pointed to some key differences in CAZyme genes and their regulation between herbaceous and woody plants.

    入藏号: WOS:000235868900014 

    PubMed ID: 16415215 

    作者识别号:

    作者 ResearcherID ORCID

    Coutinho, Pedro  C-4473-2008   

    Geisler, Matt  A-4156-2008   

    Geisler-Lee, Jane  A-9702-2012   

    Henrissat, Bernard  J-2475-2012   

    Teeri, Tuula  E-7509-2013   

    Master, Emma  O-3554-2014   

     

    ISSN: 0032-0889 

     

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    70 条,共 77

    标题: Food allergy 

    作者: Sicherer, SH (Sicherer, SH); Sampson, HA (Sampson, HA)

    来源出版物: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY  : 117  : 2  : S470-S475  DOI: 10.1016/j.jaci.2005.05.048  增刊: 1  出版年: FEB 2006  

    摘要: Food allergy, defined as an adverse immune response to food proteins, affects as many as 6% of young children and 3% to 4% of adults. Food-induced allergic reactions are responsible for a variety of symptoms involving the skin, gastrointestinal tract, and respiratory tract and might be caused by IgE-mediated and non-IgE-mediated (cellular) mechanisms. Our understanding of how food allergy represents an abrogation of normal oral tolerance is evolving. Although any food can provoke a reaction, relatively few foods are responsible for the vast majority of significant food-induced allergic reactions: milk, egg, peanuts, tree nuts, fish, and shellfish. A systematic approach to diagnosis includes a careful history, followed by laboratory studies, elimination diets, and often food challenges to confirm a diagnosis. Many food allergens have been characterized at a molecular level, which has increased our understanding of the immunopathogenesis of food allergy and might soon lead to novel diagnostic and therapeutic approaches. Currently, management of food allergies consists of educating the patient to avoid ingesting the responsible allergen and to initiate therapy in case of an unintended ingestion.

    入藏号: WOS:000235865400010 

    PubMed ID: 16455349 

    作者识别号:

    作者 ResearcherID ORCID

    Kamath, Sandip  H-9741-2012  0000-0002-5956-8552 

     

    ISSN: 0091-6749 

     

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    71 条,共 77

    标题: Significance of inducible defense-related proteins in infected plants 

    作者: van Loon, LC (van Loon, L. C.); Rep, M (Rep, M.); Pieterse, CMJ (Pieterse, C. M. J.)

    来源出版物: ANNUAL REVIEW OF PHYTOPATHOLOGY  丛书: Annual Review of Phytopathology  : 44  : 135-162  DOI: 10.1146/annurev.phyto.44.070505.143425  出版年: 2006  

    摘要: Inducible defense-related proteins have been described in many plant species upon infection with oomycetes, fungi, bacteria, or viruses, or insect attack. Several types of proteins are common and have been classified into 17 families of pathogenesis-related proteins (PRs). Others have so far been found to occur more specifically in some plant species. Most PRs and related proteins are induced through the action of the signaling compounds salicylic acid, jasmonic acid, or ethylene, and possess antimicrobial activities in vitro through hydrolytic activities on cell walls, contact toxicity, and perhaps an involvement in defense signaling. However, when expressed in transgenic plants, they reduce only a limited number of diseases, depending on the nature of the protein, plant species, and pathogen involved. As exemplified by the PR-1 proteins in Arabidopsis and rice, many homologous proteins belonging to the same family are regulated developmentally and may serve different functions in specific organs or tissues. Several defense-related proteins are induced during senescence, wounding or cold stress, and some possess antifreeze activity. Many defense-related proteins are present constitutively in floral tissues and a substantial number of PR-like proteins in pollen, fruits, and vegetables can provoke allergy in humans. The evolutionary conservation of similar defense-related proteins in monocots and dicots, but also their divergent occurrence in other conditions, suggest that these proteins serve essential functions in plant life, whether in defense or not.

    入藏号: WOS:000241126300007 

    PubMed ID: 16602946 

    作者识别号:

    作者 ResearcherID ORCID

    Utrecht Univ., PMI  G-9549-2011   

    Pieterse, Corne  A-9326-2011  0000-0002-5473-4646 

    allasia, valerie  B-4214-2009   

     

    ISSN: 0066-4286 

    ISBN: 978-0-8243-1344-9

     

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    72 条,共 77

    标题: Seduced by the dark side: integrating molecular and ecological perspectives on the inflence of light on plant defence against pests and pathogens 

    作者: Roberts, MR (Roberts, MR); Paul, ND (Paul, ND)

    来源出版物: NEW PHYTOLOGIST  : 170  : 4  : 677-699  DOI: 10.1111/j.1469-8137.2006.01707.x  出版年: 2006  

    摘要: Plants frequently suffer attack from herbivores and microbial pathogens, and have evolved a complex array of defence mechanisms to resist defoliation and disease. These include both preformed defences, ranging from structural features to stores of toxic secondary metabolites, and inducible defences, which are activated only after an attack is detected. It is well known that plant defences against pests and pathogens are commonly affected by environmental conditions, but the mechanisms by which responses to the biotic and abiotic environments interact are only poorly understood. In this review, we consider the impact of light on plant defence, in terms of both plant life histories and rapid scale molecular responses to biotic attack. We bring together evidence that illustrates that light not only modulates defence responses via its influence on biochemistry and plant development but, in some cases, is essential for the development of resistance. We suggest that the interaction between the light environment and plant defence is multifaceted, and extends across different temporal and biological scales.

    入藏号: WOS:000237353100007 

    PubMed ID: 16684231 

    作者识别号:

    作者 ResearcherID ORCID

    Roberts, Michael  H-2664-2012  0000-0002-1441-7363 

    Paul, Nigel  E-5350-2014  0000-0001-6959-4239 

     

    ISSN: 0028-646X 

     

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    73 条,共 77

    标题: The family 10 of plant pathogenesis-related proteins: Their structure, regulation, and function in response to biotic and abiotic stresses 

    作者: Liu, JJ (Liu, Jun-Jun); Ekramoddoullah, AKM (Ekramoddoullah, Abul K. M.)

    来源出版物: PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY  : 68  : 1-3  : 3-13  DOI: 10.1016/j.pmpp.2006.06.004  出版年: JAN-MAR 2006  

    摘要: The aim of this review is to present the current state of our understanding on the structure, regulation, and function of plant pathogenesis-related protein family 10 (PR-10). This protein family consists of relatively diverse members subgrouped into classes that suggest different functions. It is believed that PR-10 proteins are involved in plant defense because their genes are usually induced upon the attack of various pathogens and by environmental stresses. However, updated evidence shows that PR-10 proteins display several additional functions, including a role in developmental processes and enzymatic activities in secondary metabolism. Because of the complexity of the PR-10 gene family and its potential multiple functions, it is important to summarize current knowledge as basis for a further dissection of the functions of PR-10 proteins and a better understanding of their structural adaptation. This paper provides the first review of existing knowledge of plant PR-10 proteins and examines their structural and functional adaptations. (c) 2006 Elsevier Ltd. All rights reserved.

    入藏号: WOS:000242256300002 

    ISSN: 0885-5765 

     

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    74 条,共 77

    标题: Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis 

    作者: Andersson-Gunneras, S (Andersson-Gunneras, S); Mellerowicz, EJ (Mellerowicz, EJ); Love, J (Love, J); Segerman, B (Segerman, B); Ohmiya, Y (Ohmiya, Y); Coutinho, PM (Coutinho, PM); Nilsson, P (Nilsson, P); Henrissat, B (Henrissat, B); Moritz, T (Moritz, T); Sundberg, B (Sundberg, B)

    来源出版物: PLANT JOURNAL  : 45  : 2  : 144-165  DOI: 10.1111/j.1365-313X.2005.02584.x  出版年: JAN 2006  

    摘要: Stems and branches of angiosperm trees form tension wood (TW) when exposed to a gravitational stimulus. One of the main characteristics of TW, which distinguishes it from normal wood, is the formation of fibers with a thick inner gelatinous cell wall layer mainly composed of crystalline cellulose. Hence TW is enriched in cellulose, and deficient in lignin and hemicelluloses. An expressed sequence tag library made from TW-forming tissues in Populus tremula (L.) x tremuloides (Michx.) and data from transcript profiling using microarray and metabolite analysis were obtained during TW formation in Populus tremula (L.) in two growing seasons. The data were examined with the aim of identifying the genes responsible for the change in carbon (C) flow into various cell wall components, and the mechanisms important for the formation of the gelatinous cell wall layer (G-layer). A specific effort was made to identify carbohydrate-active enzymes with a putative function in cell wall biosynthesis. An increased C flux to cellulose was suggested by a higher abundance of sucrose synthase transcripts. However, genes related to the cellulose biosynthetic machinery were not generally affected, although the expression of secondary wall-specific CesA genes was modified in both directions. Other pathways for which the data suggested increased activity included lipid and glucosamine biosynthesis and the pectin degradation machinery. In addition, transcripts encoding fasciclin-like arabinogalactan proteins were particularly increased and found to lack true Arabidopsis orthologs. Major pathways for which the transcriptome and metabolome analysis suggested decreased activity were the pathway for C flux through guanosine 5'-diphosphate (GDP) sugars to mannans, the pentose phosphate pathway, lignin biosynthesis, and biosynthesis of cell wall matrix carbohydrates. Several differentially expressed auxin- and ethylene-related genes and transcription factors were also identified.

    入藏号: WOS:000234129600002 

    PubMed ID: 16367961 

    作者识别号:

    作者 ResearcherID ORCID

    Coutinho, Pedro  C-4473-2008   

    Nilsson, Peter  E-4547-2010  0000-0002-4657-8532 

    Henrissat, Bernard  J-2475-2012   

     

    ISSN: 0960-7412 

     

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    75 条,共 77

    标题: Growth of the plant cell wall 

    作者: Cosgrove, DJ (Cosgrove, DJ)

    来源出版物: NATURE REVIEWS MOLECULAR CELL BIOLOGY  : 6  : 11  : 850-861  DOI: 10.1038/nrm1746  出版年: NOV 2005  

    摘要: Plant cells encase themselves within a complex polysaccharide wall, which constitutes the raw material that is used to manufacture textiles, paper, lumber, films, thickeners and other products. The plant cell wall is also the primary source of cellulose, the most abundant and useful biopolymer on the Earth. The cell wall not only strengthens the plant body, but also has key roles in plant growth, cell differentiation, intercellular communication, water movement and defence. Recent discoveries have uncovered how plant cells synthesize wall polysaccharides, assemble them into a strong fibrous network and regulate wall expansion during cell growth.

    入藏号: WOS:000233194900013 

    PubMed ID: 16261190 

    ISSN: 1471-0072 

     

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    76 条,共 77

    标题: EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis 

    作者: Goicoechea, M (Goicoechea, M); Lacombe, E (Lacombe, E); Legay, S (Legay, S); Mihaljevic, S (Mihaljevic, S); Rech, P (Rech, P); Jauneau, A (Jauneau, A); Lapierre, C (Lapierre, C); Pollet, B (Pollet, B); Verhaegen, D (Verhaegen, D); Chaubet-Gigot, N (Chaubet-Gigot, N); Grima-Pettenati, J (Grima-Pettenati, J)

    来源出版物: PLANT JOURNAL  : 43  : 4  : 553-567  DOI: 10.1111/j.1365-313X.2005.02480.x  出版年: AUG 2005  

    摘要: EgMYB2, a member of a new subgroup of the R2R3 MYB family of transcription factors, was cloned from a library consisting of RNA from differentiating Eucalyptus xylem. EgMYB2 maps to a unique locus on the Eucalyptus grandis linkage map and co-localizes with a quantitative trait locus (QTL) for lignin content. Recombinant EgMYB2 protein was able to bind specifically the cis-regulatory regions of the promoters of two lignin biosynthetic genes, cinnamoyl-coenzyme A reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD), which contain MYB consensus binding sites. EgMYB2 was also able to regulate their transcription in both transient and stable expression assays. Transgenic tobacco plants over-expressing EgMYB2 displayed phenotypic changes relative to wild-type plants, among which were a dramatic increase in secondary cell wall thickness, and an alteration of the lignin profiles. Transcript abundance of genes encoding enzymes specific to lignin biosynthesis was increased to varying extents according to the position of individual genes in the pathway, whereas core phenylpropanoid genes were not significantly affected. Together these results suggest a role for EgMYB2 in the co-ordinated control of genes belonging to the monolignol-specific pathway, and therefore in the biosynthesis of lignin and the regulation of secondary cell wall formation.

    入藏号: WOS:000230889200008 

    PubMed ID: 16098109 

    ISSN: 0960-7412 

     

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    77 条,共 77

    标题: CINNAMYL ALCOHOL DEHYDROGENASE-C and -D are the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis 

    作者: Sibout, R (Sibout, R); Eudes, A (Eudes, A); Mouille, G (Mouille, G); Pollet, B (Pollet, B); Lapierre, C (Lapierre, C); Jouanin, L (Jouanin, L); Seguin, A (Seguin, A)

    来源出版物: PLANT CELL  : 17  : 7  : 2059-2076  DOI: 10.1105/tpc.105.030767  出版年: JUL 2005  

    摘要: During lignin biosynthesis in angiosperms, coniferyl and sinapyl aldehydes are believed to be converted into their corresponding alcohols by cinnamyl alcohol dehydrogenase (CAD) and by sinapyl alcohol dehydrogenase (SAD), respectively. This work clearly shows that CAD-C and CAD-D act as the primary genes involved in lignin biosynthesis in the floral stem of Arabidopsis thaliana by supplying both coniferyl and sinapyl alcohols. An Arabidopsis CAD double mutant (cad-c cad-d) resulted in a phenotype with a limp floral stem at maturity as well as modifications in the pattern of lignin staining. Lignin content of the mutant stem was reduced by 40%, with a 94% reduction, relative to the wild type, in conventional beta-O-4-linked guaiacyl and syringyl units and incorportion of coniferyl and sinapyl aldehydes. Fourier transform infrared spectroscopy demonstrated that both xylem vessels and fibers were affected. GeneChip data and real-time PCR analysis revealed that transcription of CAD homologs and other genes mainly involved in cell wall integrity were also altered in the double mutant. In addition, molecular complementation of the double mutant by tissue-specific expression of CAD derived from various species suggests different abilities of these genes/proteins to produce syringyl-lignin moieties but does not indicate a requirement for any specific SAD gene.

    入藏号: WOS:000230165400017 

    PubMed ID: 15937231 

    ISSN: 1040-4651 

     

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